FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate1_32.3520000005b692.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate1_32.3520000005b692.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59541
Sequences flagged as poor quality0
Sequence length33
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTGAATGGGCTAACTATCCAC7151.200853193597689No Hit
CTATCCACGTACTCAAAAAAAAAAAAAAAAAAA7061.1857375589929628No Hit
GTGAATGGGCTAACTATCCACAAAAAAAAAAAA5870.9858752792193615No Hit
GTATCAACGCAGAGTGAATGGGCTAACTATCCA4630.7776154246653566No Hit
GGCTAACTATCCACGTACTCTGCGTTGATACCA4000.6718059824322735No Hit
ATCCACGTACTCAAAAAAAAAAAAAAAAAAAAA3980.6684469525201121No Hit
ACGCAGAGTGAATGGGCTAACTATCCACAAAAA3890.653331317915386No Hit
GGCTAACTATCCACAAAAAAAAAAAAAAAAAAA3140.5273676962093348No Hit
CTATCCACAAAAAAAAAAAAAAAAAAAAAAAAA3050.5122520616046086No Hit
ATCCACAAAAAAAAAAAAAAAAAAAAAAAAAAA2720.456828068053946No Hit
GTGAATGGGCTAACTATCCACGTACTCAAAAAA2670.4484304932735426No Hit
GAGTGAATGGGCTAACTATCCACAAAAAAAAAA2400.4030835894593641No Hit
TATCAACGCAGAGTGAATGGGGCTAACTATCCA2390.40140407450328347No Hit
GTGAATGGGGCTAACTATCCACAAAAAAAAAAA2230.37453183520599254No Hit
ACGCAGAGTGAATGGGCTAACTATCCACGTACT2180.36613426042558905No Hit
GTGAATGGGGCTAACTATCCACGTACTCAAAAA2100.3526981407769436No Hit
AACGCAGAGTGAATGGGCTAACTATCCACGTAC1970.33086444634789475No Hit
GTGAATGGGCTAACTATCCACGTACAAAAAAAA1910.3207873566114106No Hit
ATCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1740.29223560235803897No Hit
AACGCAGAGTGAATGGGCTAACTATCCACAAAA1740.29223560235803897No Hit
GGCTAACTATCCACGTACTCAAAAAAAAAAAAA1660.27879948270939353No Hit
GTGAATGGGCTAACTATCCAAAAAAAAAAAAAA1580.26536336306074804No Hit
CTATCCACGTACAAAAAAAAAAAAAAAAAAAAA1540.2586453032364253No Hit
ACTATCCACAAAAAAAAAAAAAAAAAAAAAAAA1440.24185015367561846No Hit
GCTAACTATCCACGTACTCTGCGTTGATACCAC1410.23681160880737645No Hit
GGTATCAACGCAGAGTGAATGGGCTAACTATCC1390.23345257889521506No Hit
CGTACTCAAAAAAAAAAAAAAAAAAAAAAAAAA1220.20490082464184345No Hit
CTATCCAAAAAAAAAAAAAAAAAAAAAAAAAAA1190.19986227977360138No Hit
ATCAACGCAGAGTGAATGGGCTAACTATCCACG1170.19650324986144No Hit
CTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1160.19482373490535934No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1150.19314421994927863No Hit
TATCTACGCAGAGTGAATGGGCTAACTATCCAC1150.19314421994927863No Hit
CGTACAAAAAAAAAAAAAAAAAAAAAAAAAAAA1140.19146470499319795No Hit
GTATCATCGCAGAGTGAATGGGCTAACTATCCA1130.1897851900371173No Hit
ACGCAGAGTGAATGGGGCTAACTATCCACGTAC1110.18642616012495591No Hit
ACGCAGAGTGAATGGGCTAACTATCCACGTACA1110.18642616012495591No Hit
ACGCAGAGTGAATGGGCTAACTATCCAAAAAAA1080.18138761525671387No Hit
GTGAATGGGCTAACTAAAAAAAAAAAAAAAAAA1080.18138761525671387No Hit
GAGTGAATGGGGCTAACTATCCACAAAAAAAAA1080.18138761525671387No Hit
ATCAACGCAGAGTGAATGGGCTAACTATCCACA1060.1780285853445525No Hit
GAGTGAATGGGCTAACTATCCACGTACTCAAAA1050.1763490703884718No Hit
ATCCACGTACAAAAAAAAAAAAAAAAAAAAAAA1050.1763490703884718No Hit
GTATCAACGCAGAGTGAATGGGGCTAACTATCC1010.16963101056414906No Hit
GGCTAACTATCCACGTACAAAAAAAAAAAAAAA1010.16963101056414906No Hit
TATCAACGCAGAGTGAATGGGCTAACTATCCAA990.16627198065198773No Hit
ACTATCCACGTACTCAAAAAAAAAAAAAAAAAA950.15955392082766495No Hit
GTGAATGGGGCTAACTAAAAAAAAAAAAAAAAA940.1578744058715843No Hit
GAGTGAATGGGCTAACTATCCACGTACAAAAAA930.1561948909155036No Hit
GAATGGGCTAACTATCCACAAAAAAAAAAAAAA920.1545153759594229No Hit
GAGTGAATGGGGCTAACTATCCACGTACTCAAA880.1477973161351002No Hit
GTGAATGGGCTAACTATAAAAAAAAAAAAAAAA840.14107925631077745No Hit
GAATGGGGCTAACTATCCACAAAAAAAAAAAAA840.14107925631077745No Hit
GGGCTAACTATCCACAAAAAAAAAAAAAAAAAA800.13436119648645473No Hit
CAACGCAGAGTGAATGGGCTAACTATCCACAAA730.12260459179388992No Hit
GTGAATGGGGCTAACTATCCACGTACAAAAAAA720.12092507683780923No Hit
GCTAACTATCCACGTACTCAAAAAAAAAAAAAA710.11924556188172857No Hit
CAACGCAGAGTGAATGGGCTAACTATCCACGTA710.11924556188172857No Hit
GGGCTAACTATCCACGTACTCTGCGTTGATACC700.11756604692564787No Hit
GAGTGAATGGGGCTAACTAAAAAAAAAAAAAAA660.11084798710132514No Hit
CGGCTAACTATCCACGTACTCTGCGTTGATACC640.10748895718916376No Hit
GAGTGAATGGGCTAACTATCCAAAAAAAAAAAA630.10580944223308308No Hit
GTACTCAAAAAAAAAAAAAAAAAAAAAAAAAAA620.1041299272770024No Hit
GTGAATGGGGCTAACTATCCAAAAAAAAAAAAA620.1041299272770024No Hit
GGGCTAACTATCCACGTACTCAAAAAAAAAAAA610.10245041232092172No Hit
GCTAACTATCCACAAAAAAAAAAAAAAAAAAAA610.10245041232092172No Hit
CTAACTATCCACGTACTCAAAAAAAAAAAAAAA600.10077089736484103No Hit
ACGCAGAGTGAATGGGGCTAACTATCCACAAAA600.10077089736484103No Hit
ATCAACGCAGAGTGAATGGGGCTAACTATCCAC600.10077089736484103No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCTA200.00856941626.9848631
GTATCAT651.200533E-1024.9091031
TATCATC651.200533E-1024.9091032
CAACTCA451.661052E-623.9865441
CTCAGAG451.661052E-623.9865444
TCGCAGA752.7284841E-1123.3868797
ATCGCAG752.7284841E-1123.3868796
CTGAGTG352.0152218E-423.1298794
ACGCTGA352.0152218E-423.1298791
ATCATCG703.0195224E-1023.1298793
TCATCGC703.0195224E-1023.1298794
TGAGTGA352.0152218E-423.1298795
CATCGCA703.0195224E-1023.1298795
ATGGGAC300.00224694722.4873855
AACTCAG504.148289E-621.5878892
ACTCAGA504.148289E-621.5878893
AACGTAG350.005490423619.27491
CCCGCGG350.005490423619.27494
TACGCAG501.0182861E-418.8894025
TAGAGTG450.001097605717.9899085
Produced by FastQC (version 0.11.2)