FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate1_31.3520000005b66b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate1_31.3520000005b66b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences64338
Sequences flagged as poor quality0
Sequence length33
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGAATGGGATGTCTGACGTACTCAAAAAAAAA2870.4460816313842519No Hit
GTGAATGGGACATCGCAAAAAAAAAAAAAAAAA2220.3450526904784109No Hit
GTGAATGGGGACATCGCAAAAAAAAAAAAAAAA1930.2999782399204203No Hit
TATCAACGCAGAGTGAATGGGATGTCTGACGTA1800.2797724517392521No Hit
TGTCTGACGTACTCAAAAAAAAAAAAAAAAAAA1590.24713233236967266No Hit
GGGTCAGACATCGCAAAAAAAAAAAAAAAAAAA1440.22381796139140167No Hit
ACGTCAGACATCGCAAAAAAAAAAAAAAAAAAA1370.21293792160154185No Hit
GAGTGAATGGGATGTCTGACGTACTCAAAAAAA1270.19739500761602785No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1250.19428642481892505No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA1230.19117784202182225No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA1120.17408063663775683No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA1090.16941776244210263No Hit
ACGCAGAGTGAATGGGATGTCTGACGTACTCAA1060.16475488824644846No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT1010.15698343125369146No Hit
GACGTACTCAAAAAAAAAAAAAAAAAAAAAAAA970.15076626565948584No Hit
GTATCAACGCAGAGTGAATGGGATGTCTGACGT970.15076626565948584No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA920.14299480866672884No Hit
GAATGGGGACATCGCAAAAAAAAAAAAAAAAAA910.14144051726817744No Hit
GAGTGAATGGGAAGCAGTGGTATCAAAAAAAAA900.13988622586962604No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA900.13988622586962604No Hit
GTCTGACGTACTCAAAAAAAAAAAAAAAAAAAA860.13366906027542044No Hit
GAATGGGACATCGCAAAAAAAAAAAAAAAAAAA820.12745189468121482No Hit
GAGTGAATGGGGACATCGCAAAAAAAAAAAAAA790.12278902048556063No Hit
GTCAGACATCGCAAAAAAAAAAAAAAAAAAAAA760.11812614628990645No Hit
GACATCGCAAAAAAAAAAAAAAAAAAAAAAAAA750.11657185489135503No Hit
GAGTACGTCAGACATCGCAAAAAAAAAAAAAAA740.11501756349280363No Hit
GTGAATGGGGAGTACGTCAGACATCGCAAAAAA740.11501756349280363No Hit
CATCGCAAAAAAAAAAAAAAAAAAAAAAAAAAA730.11346327209425222No Hit
CTGACGTACTCAAAAAAAAAAAAAAAAAAAAAA730.11346327209425222No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA710.11035468929714942No Hit
GTTGCGATGTCTGACGTACTCTGCGTTGATACC710.11035468929714942No Hit
AACGCAGAGTGAATGGGATGTCTGACGTACTCA700.10880039789859804No Hit
ACGCAGAGTGAATGGGGACATCGCAAAAAAAAA690.10724610650004662No Hit
GGTCAGACATCGCAAAAAAAAAAAAAAAAAAAA670.10413752370294384No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTACG405.9400554E-726.9953272
GTATCAT356.464619E-626.9953271
CACCTTG200.00855823726.9953276
TATCTAC405.9400554E-726.9953271
ATCATCG356.464619E-626.9953273
TGTGCTC200.00855823726.99532710
TCTACGC451.6589129E-623.9958463
CTACGCA451.6589129E-623.9958464
TATCATC401.8209972E-523.620912
TCGCAGA401.8209972E-523.620917
ATCGCAG401.8209972E-523.620916
CTTACGT352.0123762E-423.13885120
TTACGTA352.0123762E-423.13885121
TCTTACG352.0123762E-423.13885119
TACGCAG553.8110193E-722.0870865
GTCTTAC404.962552E-420.24649418
ATGTCTT404.962552E-420.24649416
CAGACGT404.962552E-420.2464944
TGTCTTA450.001096054317.99688517
TACGTAC500.002207677816.20982222
Produced by FastQC (version 0.11.2)