FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate1_28.3520000005b60e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate1_28.3520000005b60e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences84026
Sequences flagged as poor quality0
Sequence length33
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTCGTCCTCTACGTACTCTGCGTTGATACCA7360.8759193582938615No Hit
CTCGTCCTCTACGTACTCTGCGTTGATACCACT3010.35822245495441885No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA2850.3391807297741175No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT2810.3344202984790422No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA2770.32965986718396684No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA2740.32608954371266036No Hit
GTGAATGGGATCTCGTCCAAAAAAAAAAAAAAA2510.29871706376597723No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA2250.2677742603479875No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA2120.2523028586389927No Hit
GAGTGAATGGGAAGCAGTGGTATCAAAAAAAAA1930.22969080998738486No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1710.20350843786447054No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA1600.19041725180301336No Hit
GAGTGAATGGGATCTCGTCCAAAAAAAAAAAAA1550.18446671268416917No Hit
CATCTCGTCCTCTACGTACTCTGCGTTGATACC1500.17851617356532504No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA1340.1594744483850237No Hit
GTGAATGGGATCTCGTCAAAAAAAAAAAAAAAA1320.15709423273748602No Hit
TATCAACGCAGAGTGAATGGGATCTCGTCCAAA1290.1535239092661795No Hit
AACGCAGAGTGAATGGGAAGCAGTGGTATCAAA1160.13805250755718468No Hit
ACGCAGAGTGAATGGGATCTCGTCCAAAAAAAA1100.1309118606145717No Hit
GTGAATGGGATCTCGTCCTCAAAAAAAAAAAAA1060.12615142931949636No Hit
CCCCCATCTCGTCCTCTACGTACTCTGCGTTGA900.10710970413919502No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAT200.00857223226.9958291
GGGGAGG200.00857223226.9958297
TACGCAG352.0193258E-423.1392865
CCTCATC300.002249317722.4965273
ATACCAC608.864663E-720.24687426
TGGGGAG404.979496E-420.2468726
CTACGCA404.979496E-420.2468724
CTAAATC350.005496039519.2827383
AGCATCT350.005496039519.2827386
GCTCATC350.005496039519.2827383
TATCTAC501.02149635E-418.8970811
TACCACT651.9089493E-618.68942327
TCTACGC450.001099753517.9972213
CTCATCT1052.577508E-916.7117044
TAAATCT500.002226626716.1974984
ATCTACG550.00420197214.72499752
GCGTTGA6300.014.56917927
CAAATCT600.007482336413.4979164
GATACCA1809.094947E-1213.49791527
TATCAAC4450.013.3462521
Produced by FastQC (version 0.11.2)