FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate1_27.3520000005b5e3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate1_27.3520000005b5e3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences146513
Sequences flagged as poor quality0
Sequence length33
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA10340.7057394224403295No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6300.42999597305358567No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA4510.30782251404312244No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA4030.2750609160961826No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA4020.2743783828056213No Hit
GGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAA3590.2450294513114877No Hit
GTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA3450.23547398524363028No Hit
GAGTGAATGGGAAGCAGTGGTATCAAAAAAAAA3360.22933118562857902No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT2880.19656958768163915No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA2410.16449052302526054No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA2390.16312545644413806No Hit
GAGTACGTTTCGACCAAAAAAAAAAAAAAAAAA2350.16039532328189307No Hit
GGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2320.15834772341020933No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA2160.14742719076122937No Hit
TATCAACGCAGAGTGAATGGGTCGAAACGTACT1830.12490359217270822No Hit
GTCGAAACGTACTCAAAAAAAAAAAAAAAAAAA1670.11398305952372828No Hit
GGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAA1660.11330052623316701No Hit
GGTCGAAACGTACTCAAAAAAAAAAAAAAAAAA1640.11193545965204454No Hit
GTACGTTTCGACCAAAAAAAAAAAAAAAAAAAA1610.10988785978036078No Hit
TCGTTGGTCGAAACGTACTCTGCGTTGATACCA1530.10442759345587081No Hit
ATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAA1500.10237999358418708No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTTGAA200.00861227426.9832612
TTGAAAC200.00861227426.9832614
TACGCAG1000.024.2849335
CTACGCA950.024.1429184
ATCTACG900.023.9851192
TCTACGC900.023.9851193
TTACTCT352.0029694E-423.18395222
TATCTAC1000.022.9436071
GTATCAT950.022.7305131
ACGCTGA1300.021.8016191
CTGAGTG1300.021.794174
GCTGAGT1300.021.794173
CGCTGAG1350.020.986982
TGAGTGA1350.020.986985
CAACTCA851.5643309E-1020.6413081
GTAGAGT608.998013E-720.2374444
CGTAGAG608.998013E-720.2374443
TAGAGTG608.998013E-720.2374445
TATCATC1100.019.6241872
CAACTCG350.005530094719.2737585
Produced by FastQC (version 0.11.2)