FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate1_22.3520000005b550.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate1_22.3520000005b550.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences81328
Sequences flagged as poor quality0
Sequence length33
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGAATGGGTAGTTCTGACACAAAAAAAAAAAA9531.1717981506984065No Hit
TATCAACGCAGAGTGAATGGGTAGTTCTGACAC7620.9369466850285264No Hit
GTTCTGACACGTACAAAAAAAAAAAAAAAAAAA5440.6688963210702341No Hit
ACGCAGAGTGAATGGGTAGTTCTGACACAAAAA4900.6024985244934093No Hit
GTATCAACGCAGAGTGAATGGGTAGTTCTGACA4160.5115089514066496No Hit
CGGTAGTTCTGACACGTACTCTGCGTTGATACC3950.48568758607121776No Hit
GGGTAGTTCTGACACGTACTCTGCGTTGATACC3610.44388156600432815No Hit
GTGAATGGGGTAGTTCTGACACAAAAAAAAAAA3380.41560102301790286No Hit
GTGAATGGGGTAGTTCAAAAAAAAAAAAAAAAA3060.3762541806020067No Hit
GGGTAGTTCTGACACAAAAAAAAAAAAAAAAAA2980.36641746999803265No Hit
GTTCTGACACGTACTCAAAAAAAAAAAAAAAAA2870.35289199291756834No Hit
AACGCAGAGTGAATGGGTAGTTCTGACACAAAA2500.30739720637418844No Hit
GTTCTGACACAAAAAAAAAAAAAAAAAAAAAAA2460.30247885107220146No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2290.28157584103875666No Hit
CGTACAAAAAAAAAAAAAAAAAAAAAAAAAAAA2260.2778870745622664No Hit
TATCAACGCAGAGTGAATGGGGTAGTTCTGACA2230.27419830808577617No Hit
GTGAATGGGTAGTTCTGACACGTACAAAAAAAA2180.26805036395829235No Hit
GAGTGAATGGGTAGTTCTGACACAAAAAAAAAA1850.22747393271689947No Hit
GTGAATGGGTAGTTCTGACACGAAAAAAAAAAA1830.22501475506590596No Hit
GTGAATGGGTAGTTCAAAAAAAAAAAAAAAAAA1780.21886681093842222No Hit
GTGAATGGGGTAGTTCTGACACGTACAAAAAAA1650.2028821562069644No Hit
TTCTGACACAAAAAAAAAAAAAAAAAAAAAAAA1550.19058626795199685No Hit
GTATCAACGCAGAGTGAATGGGGTAGTTCTGAC1400.17214243556954553No Hit
GAATGGGTAGTTCTGACACAAAAAAAAAAAAAA1390.17091284674404877No Hit
GGTATCAACGCAGAGTGAATGGGTAGTTCTGAC1310.16107613614007474No Hit
AGGTAGTTCTGACACGTACTCTGCGTTGATACC1310.16107613614007474No Hit
GTATCATCGCAGAGTGAATGGGTAGTTCTGACA1310.16107613614007474No Hit
CACGTACAAAAAAAAAAAAAAAAAAAAAAAAAA1290.15861695848908125No Hit
GTGAATGGGTAGTTCTGACAAAAAAAAAAAAAA1280.1573873696635845No Hit
GTTCTGACACGAAAAAAAAAAAAAAAAAAAAAA1210.14878024788510721No Hit
GTTCTGACACGTAAAAAAAAAAAAAAAAAAAAA1130.1389435372811332No Hit
GAGTGAATGGGGTAGTTCTGACACAAAAAAAAA1130.1389435372811332No Hit
ACGCAGAGTGAATGGGTAGTTCTGACACGAAAA1120.13771394845563642No Hit
TATCAACGCAGAGTGAATGGGGTAGTTCAAAAA1100.13525477080464293No Hit
GAGTGAATGGGGTAGTTCAAAAAAAAAAAAAAA1080.13279559315364942No Hit
CGTACTCAAAAAAAAAAAAAAAAAAAAAAAAAA1060.1303364155026559No Hit
GTGAATGGGTAGTTCTGACACGTACTCAAAAAA1040.1278772378516624No Hit
ATCAACGCAGAGTGAATGGGTAGTTCTGACACG1030.12664764902616565No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA1010.12418847137517215No Hit
ACGCAGAGTGAATGGGGTAGTTCAAAAAAAAAA1010.12418847137517215No Hit
ACGCAGAGTGAATGGGTAGTTCTGACACGTACA1010.12418847137517215No Hit
ATCAACGCAGAGTGAATGGGTAGTTCTGACACA1000.12295888254967538No Hit
TATCTACGCAGAGTGAATGGGTAGTTCTGACAC990.12172929372417864No Hit
AACGCAGAGTGAATGGGTAGTTCTGACACGTAC980.12049970489868189No Hit
ACGCAGAGTGAATGGGTAGTTCTGACACGTACT980.12049970489868189No Hit
GACACAAAAAAAAAAAAAAAAAAAAAAAAAAAA970.11927011607318512No Hit
GTGAATGGGTAGTTCTGACACGTAAAAAAAAAA970.11927011607318512No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA950.11681093842219163No Hit
GTGAATGGGGTAGTTCTGACACGTACTCAAAAA940.11558134959669486No Hit
GTACTCAAAAAAAAAAAAAAAAAAAAAAAAAAA920.11312217194570137No Hit
CAACGCAGAGTGAATGGGTAGTTCTGACACAAA910.1118925831202046No Hit
CCCCCCGGTAGTTCTGACACGTACTCTGCGTTG910.1118925831202046No Hit
GAATGGGGTAGTTCAAAAAAAAAAAAAAAAAAA900.11066299429470786No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA900.11066299429470786No Hit
ACGCAGAGTGAATGGGGTAGTTCTGACACGTAC900.11066299429470786No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT890.10943340546921111No Hit
TGGTAGTTCTGACACGTACTCTGCGTTGATACC850.10451505016722408No Hit
GGGTAGTTCTGACACGTACAAAAAAAAAAAAAA850.10451505016722408No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGTAG307.0759306E-527.0012321
GTATCTA200.00856221427.0012321
GTATCAT307.0759306E-527.0012321
GTAGAGT257.790871E-426.9846064
ACGTAGA307.105713E-526.9846062
CCTTGGT200.00858800526.9846065
CGTAGAG307.105713E-526.9846063
GTGGGGT200.00858800526.9846065
TAGAGTG257.790871E-426.9846065
CAACTCA401.825632E-523.626081
TATCATC352.024228E-423.1296622
AAACAGC300.00224606222.5010281
GAGTAAA300.00224606222.5010281
TGTGGTA300.002254127522.4871736
TTACTCT504.091162E-621.64098722
TGACACT658.306233E-820.75738716
ACACTTA608.89273E-720.23845518
GACACTT608.89273E-720.23845517
ACTTACT608.89273E-720.23845520
CTTACTC608.89273E-720.23845521
Produced by FastQC (version 0.11.2)