FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate1_2.3520000005b2cc.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate1_2.3520000005b2cc.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences78207
Sequences flagged as poor quality0
Sequence length33
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA3080.3938266395591188No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA2740.3503522702571381No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA2690.3439589806539057No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT2590.33117240144744076No Hit
GAGTGAATGGGAAGCAGTGGTATCAAAAAAAAA2220.2838620583835207No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA1910.24422366284347946No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA1840.23527305739895407No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA1670.21353587274796373No Hit
CCCTAGCTCACTACCACGTACTCTGCGTTGATA1600.2045852673034383No Hit
GTAGTGAGCTAAAAAAAAAAAAAAAAAAAAAAA1300.1662255296840436No Hit
GTGGTAGTGAGCTAAAAAAAAAAAAAAAAAAAA1240.15855358216016469No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1210.15471760839822524No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA1160.14832431879499278No Hit
AACGCAGAGTGAATGGGAAGCAGTGGTATCAAA1090.13937371335046733No Hit
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCC1040.1329804237472349No Hit
GTACGTGGTAGTGAGCTAAAAAAAAAAAAAAAA900.11507921285818405No Hit
GCCTAGCTCACTACCACGTACTCTGCGTTGATA870.11124323909624458No Hit
CCCCTAGCTCACTACCACGTACTCTGCGTTGAT840.10740726533430513No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCTGA200.00859194726.9807951
GTTGATA1650.022.14608427
GTGAATA404.993938E-420.2355961
TTCCTAG350.005510390719.2719965
TGAATAG350.005510390719.2719962
CTCTGCT450.001079413618.04495827
ATGGGTG500.002233048416.1884775
AATGGGT1054.7073627E-815.4175964
CGTTGAT3450.014.12214127
ATCTAGC600.007503797813.49039656
TATCAAC3050.012.8269361
GCGTTGA6250.011.90967327
ATGGGAC1102.7344638E-411.0375985
TCTGCGT19650.010.88207427
AGTACGT1000.001539134510.7923172
GAGTACG1000.001539134510.7923171
ATCAACG3500.010.7923172
ATTAGCT1401.9823541E-510.5995978
TGCGTTG10200.010.481997527
TCAACGC3750.010.072833
Produced by FastQC (version 0.11.2)