FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate1_19.3520000005b4f1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate1_19.3520000005b4f1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences169073
Sequences flagged as poor quality0
Sequence length33
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTGAATGGGCTCTGAACGTAC16810.9942450893992536No Hit
GCTCTGAACGTACTCAAAAAAAAAAAAAAAAAA12660.748788984639771No Hit
GAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11450.6772222649388134No Hit
GAGTGAATGGGCTCTGAACGAAAAAAAAAAAAA11060.654155305696356No Hit
GTATCAACGCAGAGTGAATGGGCTCTGAACGTA10900.6446919378020145No Hit
GTGAATGGGCTCTGAACGAAAAAAAAAAAAAAA10390.6145274526388009No Hit
GTGAATGGGCTCTGAACGTACTCAAAAAAAAAA10100.597375098330307No Hit
GAGTGAATGGGCTCTGAACGTACTCAAAAAAAA9010.5329059045501056No Hit
ACGCAGAGTGAATGGGCTCTGAACGTACTCTGC7420.4388636861000869No Hit
ACGCAGAGTGAATGGGCTCTGAACGTACTCAAA7210.42644301573876375No Hit
ACGCAGAGTGAATGGGCTCTGAACGAAAAAAAA6910.40869920093687345No Hit
GAGTGAATGGGCTCTGAACAAAAAAAAAAAAAA6500.38444932070762333No Hit
GTGAATGGGCTCTGAACAAAAAAAAAAAAAAAA6470.38267493922743434No Hit
GAACGTACTCTGCGTAAAAAAAAAAAAAAAAAA6210.36729696639912934No Hit
GTGAATGGGCTCTGAACGTACAAAAAAAAAAAA6080.3596079799849769No Hit
GAGTGAATGGGCTCTGAACGTACAAAAAAAAAA5990.3542848355444098No Hit
GGGAAGCAGTGGTATCAACGCAGAGTGAATGGG5910.3495531515972391No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA5720.33831540222270856No Hit
GTATCATCGCAGAGTGAATGGGCTCTGAACGTA5560.328852034328367No Hit
TATCAACGCAGAGTGAATGGGCTCTGAACGAAA5480.3241203503811963No Hit
GTGAATGGGCTCTGAACGTAAAAAAAAAAAAAA5220.30874237755289136No Hit
ATCAACGCAGAGTGAATGGGCTCTGAACGTACT5190.3069679960727023No Hit
GAGTGAATGGGCTCTGAACGTAAAAAAAAAAAA5020.29691316768496445No Hit
GTGAATGGGCTCTGAAAAAAAAAAAAAAAAAAA4900.2898156417642084No Hit
GAGTGAATGGGCTCTGAAAAAAAAAAAAAAAAA4850.28685833929722665No Hit
GCTCTGAACGTACAAAAAAAAAAAAAAAAAAAA4810.2844924973236413No Hit
ACGCAGAGTGAATGGGCTCTGAACAAAAAAAAA4660.2756205899226961No Hit
ACGCAGAGTGAATGGGCTCTGAACGTACAAAAA4630.27384620844250707No Hit
GTACTCAAAAAAAAAAAAAAAAAAAAAAAAAAA4480.26497430104156194No Hit
TATCTACGCAGAGTGAATGGGCTCTGAACGTAC4220.24959632821325697No Hit
GAACGTACTCAAAAAAAAAAAAAAAAAAAAAAA4220.24959632821325697No Hit
AACGCAGAGTGAATGGGCTCTGAACGTACTCTG3790.22416352699721423No Hit
CTCTGAACGTACTCAAAAAAAAAAAAAAAAAAA3790.22416352699721423No Hit
GGTATCAACGCAGAGTGAATGGGCTCTGAACGT3660.21647454058306173No Hit
ACGCAGAGTGAATGGGCTCTGAAAAAAAAAAAA3630.2147001591028727No Hit
ACGCAGAGTGAATGGGCTCTGAACGTAAAAAAA3520.20819409367551298No Hit
CGTACTCAAAAAAAAAAAAAAAAAAAAAAAAAA3460.20464533071513488No Hit
TATCAACGCAGAGTGAATGGGCTCTGAACAAAA3330.19695634430098244No Hit
AACGCAGAGTGAATGGGCTCTGAACGAAAAAAA3310.19577342331418973No Hit
AACGCAGAGTGAATGGGCTCTGAACGTACTCAA3280.19399904183400069No Hit
GCTCTGAACGAAAAAAAAAAAAAAAAAAAAAAA3270.19340758134060437No Hit
GCTCTGAACGTACTCTGCGTAAAAAAAAAAAAA3190.1886758973934336No Hit
GAGTGAATGGGCTCTGAACGTACTCTGCGTAAA3080.18216983196607384No Hit
CAACTCAGAGTGAATGGGCTCTGAACGTACTCT3030.1792125294990921No Hit
GTGAATGGGCTCTGAACGTACTCTGCGTAAAAA2970.17566376653871404No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2940.173889385058525No Hit
GAACGTACAAAAAAAAAAAAAAAAAAAAAAAAA2920.17270646407173235No Hit
GTATCAACGCAGAGTGAATGGGCTCTGAACGAA2910.17211500357833598No Hit
GTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA2780.1644260171641835No Hit
GAACGAAAAAAAAAAAAAAAAAAAAAAAAAAAA2780.1644260171641835No Hit
GCTCTGAACAAAAAAAAAAAAAAAAAAAAAAAA2750.1626516356839945No Hit
AATGCGCTCTGAACGTACTCTGCGTTGATACCA2740.16206017519059815No Hit
CTGAACGTACTCTGCGTAAAAAAAAAAAAAAAA2590.153188267789653No Hit
GAATGGGCTCTGAACGAAAAAAAAAAAAAAAAA2540.15023096532267127No Hit
CGTACAAAAAAAAAAAAAAAAAAAAAAAAAAAA2520.1490480443358786No Hit
TATCAACGCAGAGTGAATGGGCTCTGAAAAAAA2500.1478651233490859No Hit
TATCAACGCAGAGTGAATGGGCTCTGAACGTAA2420.14313343940191514No Hit
GAACGTAAAAAAAAAAAAAAAAAAAAAAAAAAA2420.14313343940191514No Hit
GGCTCTGAACGTACTCAAAAAAAAAAAAAAAAA2340.1384017554547444No Hit
GCGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2290.13544445298776267No Hit
GAATGGGCTCTGAACGTACTCAAAAAAAAAAAA2280.13485299249436633No Hit
GAACGTACTCTGCGAAAAAAAAAAAAAAAAAAA2210.13071276904059195No Hit
GCTCTGAACGTACTAAAAAAAAAAAAAAAAAAA2190.12952984805379925No Hit
GAGTGAATGGGCTCTGAACGTACTCTGCGAAAA2180.1289383875604029No Hit
GGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAA2090.12361524311983582No Hit
AACGCAGAGTGAATGGGCTCTGAACAAAAAAAA2080.12302378262643947No Hit
ATCAACGCAGAGTGAATGGGCTCTGAACGAAAA2070.12243232213304313No Hit
AACGTACTCTGCGTAAAAAAAAAAAAAAAAAAA2060.12184086163964677No Hit
GTATCAACGCAGAGTGAATGGGCTCTGAACAAA2050.12124940114625044No Hit
GTGAATGGGCTCTGAACGTACTCTGCGAAAAAA2050.12124940114625044No Hit
CTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2040.1206579406528541No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA2010.11888355917266506No Hit
GCTCTGAACGTACTCTGCGAAAAAAAAAAAAAA1970.11651771719907969No Hit
AACGCAGAGTGAATGGGCTCTGAACGTACAAAA1930.11415187522549432No Hit
GAGTGAATGGGCTCTGAACGTACTCTGCGTTGA1850.10942019127832356No Hit
CTGAACGTACTCTGCGAAAAAAAAAAAAAAAAA1730.10232266535756744No Hit
GCTCTGAACGTAAAAAAAAAAAAAAAAAAAAAA1730.10232266535756744No Hit
GCTCTGAACGTACTCTGCAAAAAAAAAAAAAAA1710.10113974437077475No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT1700.10054828387737842No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCTA257.794505E-427.0032581
TTACCAA200.00862216926.979295
ATGACAA200.00862216926.979294
GTATCAT1150.025.8292051
TATCATC1200.024.7383352
GAGTAAA1100.023.3209951
CAACTCA1700.023.032191
TAAATGG1100.022.0739654
CGCTGAG806.91216E-1121.927162
TATCTAC1050.021.859781
ATCTACG1000.021.589822
TCTACGC1000.021.589823
TACGCAG1000.021.5834335
GCTGAGT707.912604E-921.2042883
AGTAAAT1150.021.1204762
ACGCTGA851.5643309E-1020.649551
CTACGCA1050.020.5556494
ACGCATA404.99903E-420.2524411
TGACAAT405.029074E-420.2344675
GTGTATG751.7382263E-819.802391
Produced by FastQC (version 0.11.2)