FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate1_17.3520000005b4bd.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate1_17.3520000005b4bd.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35234
Sequences flagged as poor quality0
Sequence length33
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATACGCACGTTACGTACTCTGCGTTGATACCA3751.064312879604927No Hit
CCATACGCACGTTACGTACTCTGCGTTGATACC1900.5392518589998297No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA1660.47113583470511433No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA1540.43707782255775673No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT1530.4342396548788103No Hit
ATACGCACGTTACGTACTCTGCGTTGATACCAC1500.42572515184197085No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA1470.41721064880513137No Hit
CCCATACGCACGTTACGTACTCTGCGTTGATAC1430.40585797808934554No Hit
GCATACGCACGTTACGTACTCTGCGTTGATACC1290.3661236305840949No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA1090.3093602770051655No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA1050.2980076062893796No Hit
GAGTGAATGGGAAGCAGTGGTATCAAAAAAAAA970.2753022648578078No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA950.2696259294999149No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA700.19867173752625306No Hit
GCCATACGCACGTTACGTACTCTGCGTTGATAC690.19583356984730657No Hit
AACGCAGAGTGAATGGGAAGCAGTGGTATCAAA580.16461372537889538No Hit
ATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAA570.16177555769994892No Hit
GTCATACGCACGTTACGTACTCTGCGTTGATAC570.16177555769994892No Hit
CTCATACGCACGTTACGTACTCTGCGTTGATAC550.15609922234205595No Hit
GAATGGGAAGCAGTGGTATCAAAAAAAAAAAAA460.13055571323153772No Hit
CCCCAGGTCAGGCGGGATTACCCGCTGAGTTTA450.12771754555259127No Hit
ACATACGCACGTTACGTACTCTGCGTTGATACC440.12487937787364477No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA430.12204121019469831No Hit
GGTATCAACGCAGAGTGAATGGGAAGCAGTGGT420.11920304251575183No Hit
CATACGCACAAAAAAAAAAAAAAAAAAAAAAAA380.10785037179996596No Hit
CCCCATACGCACGTTACGTACTCTGCGTTGATA370.10501220412101947No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACCAC404.8502412E-420.27777727
GGGGTGC751.321823E-414.39924057
TGGGGTG802.2866823E-413.4992896
GATACCA1051.1699796E-512.87477927
TGGGACG750.002178189312.5993366
ATGGGGT1101.9159012E-512.272085
GGGTGCG1001.0864193E-412.149368
TGATACC1152.977902E-511.75523426
TATCAAC1502.665165E-711.6827652
GTCATAC959.733246E-411.3678237
GGTGCGT1102.637793E-411.0448729
TCTGCGT7050.010.54636227
AATGGGG2902.910383E-119.7614624
GTGAATG7500.09.7056811
TGAATGG7400.09.6546762
CGTATGA1200.00684669668.98674313
GAATGGG7900.08.8729863
AATGGGA3058.1854523E-108.8394184
TTGATAC1701.559949E-48.74727627
ATGGGAC1400.00241342198.6781155
Produced by FastQC (version 0.11.2)