FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate1_13.3520000005b437.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate1_13.3520000005b437.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences66985
Sequences flagged as poor quality0
Sequence length33
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCATAGAGTCAAAAAAAAAAAAAAAAAAAAAAA4030.6016272299768605No Hit
ACCATAGAGTCACGTACTCTGCGTTGATACCAC3350.5001119653653803No Hit
CCACCATAGAGTCACGTACTCTGCGTTGATACC3220.48070463536612673No Hit
GCACCATAGAGTCAAAAAAAAAAAAAAAAAAAA2320.34634619690975593No Hit
CCATAGAGAAAAAAAAAAAAAAAAAAAAAAAAA2250.3358960961409271No Hit
CCATAGAGTAAAAAAAAAAAAAAAAAAAAAAAA2200.3284317384489065No Hit
GAGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAA2140.31947450921848175No Hit
GCACCATAGAGTCACGTACTCTGCGTTGATACC1950.29110994998880346No Hit
ACACCATAGAGTCACGTACTCTGCGTTGATACC1500.22393073076061804No Hit
CCATAGAGTCACGTACTCAAAAAAAAAAAAAAA1480.22094498768380982No Hit
GCACCATAGAGAAAAAAAAAAAAAAAAAAAAAA1440.21497350153019332No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1140.1701873553780697No Hit
CCATAGAAAAAAAAAAAAAAAAAAAAAAAAAAA1050.15675151153243264No Hit
CACCATAGAGTCACGTACTCTGCGTTGATACCA1020.15227289691722026No Hit
TATCAACGCAGAGTGAATGGGCACCATAGAGTC1020.15227289691722026No Hit
GAGTGAATGGGCACCATAGAGTCAAAAAAAAAA1010.15078002537881616No Hit
GCACCATAGAGTAAAAAAAAAAAAAAAAAAAAA980.1463014107636038No Hit
GTGAATGGGCACCATAGAGTCAAAAAAAAAAAA970.14480853922519968No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA940.1403299246099873No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA920.13734418153317907No Hit
GTGAATGGGCACCATAGAAAAAAAAAAAAAAAA920.13734418153317907No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA910.13585130999477496No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT900.13435843845637083No Hit
ACGCAGAGTGAATGGGCACCATAGAGTCAAAAA890.13286556691796672No Hit
ACGCAGAGTGAATGGGCACCATAGAGAAAAAAA870.12987982384115845No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA830.123908337687542No Hit
GAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAA820.12241546614913787No Hit
GAGTGAATGGGCACCATAGAGAAAAAAAAAAAA800.11942972307232963No Hit
GAGTGAATGGGAAGCAGTGGTATCAAAAAAAAA750.11196536538030902No Hit
TCACCATAGAGTCACGTACTCTGCGTTGATACC730.10897962230350079No Hit
GTGAATGGGGCACCATAGAGAAAAAAAAAAAAA720.10748675076509666No Hit
CCATAAAAAAAAAAAAAAAAAAAAAAAAAAAAA700.10450100768828842No Hit
GTGAATGGGCACCATAGAGAAAAAAAAAAAAAA690.1030081361498843No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA690.1030081361498843No Hit
GTATCAACGCAGAGTGAATGGGCACCATAGAGT680.1015152646114802No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGCGG200.00850619627.03068727
GTCACTT200.00856857626.99028417
GATCACC257.7668723E-426.9902846
TCACTTA200.00856857626.99028418
AGTCACT200.00856857626.99028416
GTATCAT300.002237493422.5087261
TATCATC350.005491113819.2787742
ATACCAC500.002201161616.21841227
TGATACC1800.015.76790227
TTGATAC1900.014.93801226
TCTGCGT20400.013.97910627
TATCAAC2700.013.5052361
ATGGGAG700.001320277713.4951425
TACCACC700.001320277713.4951426
TGCGTTG6850.013.02208327
CAACACC750.002219402712.5954666
ATCACCA1655.7352736E-912.2683117
CCACCAC906.32937E-411.9956825
CTCTGCG24350.011.87796126
ATGGGGA1051.7623165E-411.5672655
Produced by FastQC (version 0.11.2)