FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate1_12.3520000005b41d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate1_12.3520000005b41d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences149089
Sequences flagged as poor quality0
Sequence length33
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACGTACTCTGCGAAAAAAAAAAAAAAAAAAAA15631.0483670827492302No Hit
TATCAACGCAGAGTGAATGGGATTCACGTACTC13740.9215971667929893No Hit
GCGTAAGGATTCACGTACTCTGCGTTGATACCA13630.914219023536277No Hit
CACGTACTCTGCGTAAAAAAAAAAAAAAAAAAA13100.8786697878448444No Hit
GGATTCACGTACTCAAAAAAAAAAAAAAAAAAA9060.6076907082346786No Hit
GTATCAACGCAGAGTGAATGGGATTCACGTACT7540.505738183232834No Hit
GGATAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6950.46616450576501284No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6140.4118345417837667No Hit
GAGTGAATGGGATTCACGTACTCTGCGAAAAAA5300.35549235691432635No Hit
CTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5210.34945569424974343No Hit
GGATTCACGTACTCTGCGAAAAAAAAAAAAAAA5180.3474434733615491No Hit
GTGAATGGGATTCACGTACTCTGCGAAAAAAAA4860.3259797838874766No Hit
ATTCACGTACTCTGCGAAAAAAAAAAAAAAAAA4820.3232968227032175No Hit
GTGAATGGGATTCACGTACTCAAAAAAAAAAAA4210.28238166464326675No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA4070.27299130049836No Hit
GTGAATGGGCGTAAGGATAAAAAAAAAAAAAAA3960.26561315724164763No Hit
CTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3910.2622594557613238No Hit
ACGCAGAGTGAATGGGATTCACGTACTCTGCGA3740.2508568707282227No Hit
GATTCACGTACTCTGCGAAAAAAAAAAAAAAAA3720.2495153901360932No Hit
TTCACGTACTCTGCGAAAAAAAAAAAAAAAAAA3680.24683242895183413No Hit
AACGCAGAGTGAATGGGATTCACGTACTCTGCG3680.24683242895183413No Hit
GAGTGAATGGGATTCACGTACTCAAAAAAAAAA3540.2374420648069274No Hit
GCGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3380.22671022006989114No Hit
CACGTACTCTGCAAAAAAAAAAAAAAAAAAAAA3250.21799059622104916No Hit
GGATTCACGTACTCTGCGTAAAAAAAAAAAAAA3120.2092709723722072No Hit
ATCAACGCAGAGTGAATGGGATTCACGTACTCT3090.2072587514840129No Hit
ACGCAGAGTGAATGGGATTCACGTACTCAAAAA3060.20524653059581863No Hit
GATTCACGTACTCAAAAAAAAAAAAAAAAAAAA3050.20457579029975384No Hit
GGTATCAACGCAGAGTGAATGGGATTCACGTAC2920.1958561664509119No Hit
GTGAATGGGATTCACGTACTCTGCGTAAAAAAA2830.18981950378632897No Hit
CGTAAGGATTCACGTACTCTGCGTTGATACCAC2780.18646580230600515No Hit
GTATCATCGCAGAGTGAATGGGATTCACGTACT2780.18646580230600515No Hit
ACGCAGAGTGAATGGGATTCACGTACTCTGCGT2760.1851243217138756No Hit
GAGTGAATGGGATTCACGTACTCTGCGTAAAAA2650.17774617845716317No Hit
GGCGTAAGGATTCACGTACTCTGCGTTGATACC2550.1710387754965155No Hit
ACGCAGAGTGAATGGGCGTAAGGATAAAAAAAA2320.15561174868702587No Hit
GTGAATGGGCGTAAGGAAAAAAAAAAAAAAAAA2250.1509165666145725No Hit
ATTCACGTACTCTGCGTAAAAAAAAAAAAAAAA2240.15024582631850775No Hit
GATTCACGTACTCTGCGTAAAAAAAAAAAAAAA2230.149575086022443No Hit
GTGAATGGGGCGTAAGGATAAAAAAAAAAAAAA2180.14622138454211914No Hit
GGGAAGCAGTGGTATCAACGCAGAGTGAATGGG2130.14286768306179531No Hit
CTGCGAAAAAAAAAAAAAAAAAAAAAAAAAAAA2070.1388432412854067No Hit
TATCAACGCAGAGTGAATGGGCGTAAGGATAAA2050.13750176069327716No Hit
GAGTGAATGGGCGTAAGGATAAAAAAAAAAAAA2030.13616028010114764No Hit
GAATGGGATTCACGTACTCTGCGAAAAAAAAAA2020.13548953980508288No Hit
CACGTACTCAAAAAAAAAAAAAAAAAAAAAAAA2010.1348187995090181No Hit
GATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1990.13347731891688858No Hit
CTGCGTAAAAAAAAAAAAAAAAAAAAAAAAAAA1980.13280657862082382No Hit
CTCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1970.13213583832475906No Hit
TATCTACGCAGAGTGAATGGGATTCACGTACTC1950.1307943577326295No Hit
CTCTGCGAAAAAAAAAAAAAAAAAAAAAAAAAA1930.12945287714049997No Hit
CAACGCAGAGTGAATGGGATTCACGTACTCTGC1670.11201362944281604No Hit
GGATTCACGTACTCTGCAAAAAAAAAAAAAAAA1650.11067214885068652No Hit
GGATTCAAAAAAAAAAAAAAAAAAAAAAAAAAA1580.10597696677823312No Hit
GGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1550.10396474589003883No Hit
GCGTAAGGATAAAAAAAAAAAAAAAAAAAAAAA1550.10396474589003883No Hit
GTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA1510.10128178470577977No Hit
AACGCAGAGTGAATGGGATTCACGTACTCAAAA1510.10128178470577977No Hit
GAATGGGATTCACGTACTCAAAAAAAAAAAAAA1510.10128178470577977No Hit
GCGTTAAAAAAAAAAAAAAAAAAAAAAAAAAAA1500.10061104440971501No Hit
TTCACGTACTCTGCGTAAAAAAAAAAAAAAAAA1500.10061104440971501No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAT1500.026.9949571
TATCATC1450.026.9858822
CTTCCCA257.8194647E-426.9858822
GTGAATT257.834845E-426.9768123
TGAATTG257.834845E-426.9768124
TTAACGC257.834845E-426.9768124
TATCTAC1350.024.995331
CTACGCA1350.024.978534
TTACTCT501.5177466E-724.34457422
ATCTACG1350.023.9874522
TACGCAG1300.023.8560895
TCAACAC300.002266714122.4806793
GAATTGG300.00227114722.4731275
TCTACGC1450.022.325643
CAACTCA1700.022.231141
AACTCAG1800.020.989022
TCAGAGT1700.020.6223985
CATCGCA1700.020.6223985
ACTCAGA1900.020.5875683
CTCAGAG1900.020.5875684
Produced by FastQC (version 0.11.2)