FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate8_89.3510000006118c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate8_89.3510000006118c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1250
Sequences flagged as poor quality0
Sequence length27
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCAACCGATAGGCTGTCTCTTATACAC100.8No Hit
TCAACCGATAGGCTGTCTCTTCTACAC40.32No Hit
TCAACCGATAGGGCAACTGGTTTTTTT30.24No Hit
TCAACCGATAGGAGTCATAGTTTTTTT20.16No Hit
TCAACCGATAGGACTTTCAATTTTTTT20.16No Hit
TCAACCGATAGGTAGTGAGATTTTTTT20.16No Hit
TCAACCGATAGGAACTTTAGTTTTTTT20.16No Hit
TCAACCGATAGGCGATAGGATTTTTTT20.16No Hit
TCAACCGATAGGAAGTGTTGTCTGTCT20.16No Hit
TCAACCGATAGGGGATTGGGTTTTTTT20.16No Hit
TCAACCGATAGGCAAAATTGTTTTTTT20.16No Hit
TCAACCGATAGGGATACGACTTTTTTT20.16No Hit
TCAACCGATAGGATATTATGCTGTCTC20.16No Hit
TCAACCGATAGGGGGGTCAATTTTTTT20.16No Hit
TCAACCGATAGGCCCTGTCTCTTATAC20.16No Hit
TCAACCGATAGGAGACGAGATTTTTTT20.16No Hit
TCAACCGATAGGGCGTATGATTTTTTT20.16No Hit
TCAACCGATAGGGCCTGCGATTTTTTT20.16No Hit
TCAACCGATAGGCATCGACGTTTTTTT20.16No Hit
TCAACCGATAGGCCTGCGGATTTTTTT20.16No Hit
TCAACCGATAGGGTCTCCCGTTTTTTT20.16No Hit
TCAACCGATAGGAAATACTATTTTTTT20.16No Hit
TCAACCGATAGGGCTTTGCACTGTCTC20.16No Hit
TCAACCGATAGGCCGCACTATTTTTTT20.16No Hit
TCTACCGATAGGTCTTAAGATTTTTTT20.16No Hit
TCAACCGATAGGTTCTGTCTCTTATAC20.16No Hit
TCAACCGATAGGAAAGAAGGTTTTTTT20.16No Hit
TCAACCGATAGGCGGGTGGGTTTTTTT20.16No Hit
TCAACCGATAGGATGCAAACTTTTTTT20.16No Hit
TCAACCGATAGGGCGCTGCCTTTTTTT20.16No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTTTTT900.021.00000221
ATTTTTT301.6740938E-421.00000220
AACCGAT1050.021.03
GTTTTTT401.1890661E-621.020
ACCGATA1250.021.04
CCGATAG1250.021.05
GATAGGC401.1890661E-621.07
CAACCGA1000.020.9999982
TCAACCG1000.020.9999981
GATAGGT250.001853412620.9999987
CGATAGG1300.020.1923086
GATAGGA551.9022991E-819.0909087
Produced by FastQC (version 0.11.2)