FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate8_75.35100000060fd1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate8_75.35100000060fd1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1250
Sequences flagged as poor quality0
Sequence length27
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCGACCAACGAGGCGGGTATTTTTTT30.24No Hit
TTCGACCAACGACTGATCTGTTTTTTT20.16No Hit
TTCGACCAACGACCATCGGCTTTTTTT20.16No Hit
TTCGACCAACGACGCTGCTGTTTTTTT20.16No Hit
TTCGACCAACGAGAGTAGTGTTTTTTT20.16No Hit
TTCGACCAACGATCTGGTTCTTTTTTT20.16No Hit
TTCGACCAACGAAGGGCCGGTTTTTTT20.16No Hit
TTCGACCAACGAGGCGGCGGTTTTTTT20.16No Hit
TTCGACCAACGAGAGCGGGCTTTTTTT20.16No Hit
TTCGACCAACGAGATTGAGATTTTTTT20.16No Hit
TTCGACCAACGAGGATGGCGTTTTTTT20.16No Hit
TTCGACCAACGACTGGTGTGTTTTTTT20.16No Hit
TTCGACCAACGATTATCTCGTTTTTTT20.16No Hit
TTCGACCAACGAAGTGGGGGTTTTTTT20.16No Hit
TTCGACCAACGAGTGGGCGGTTTTTTT20.16No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTTTTT459.277028E-821.00000220
ACCAACG1200.021.0000025
GACCAAC1200.021.0000024
CAACGAT301.6740938E-421.0000027
CAACGAC301.6740938E-421.0000027
CCAACGA1250.021.06
TCGACCA1050.021.02
TTCGACC1050.021.01
CGACCAA1150.021.03
CAACGAG401.1890661E-621.07
CTTTTTT351.44566875E-521.020
TTTTTTT1000.020.99999821
ATTTTTT250.001853412620.99999820
CAACGAA250.001853412620.9999987
Produced by FastQC (version 0.11.2)