FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate8_7.35100000060753.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate8_7.35100000060753.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37115
Sequences flagged as poor quality0
Sequence length27
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCAGCAATGCCTGTCTCTTATACAC1550.41762090798868384No Hit
CTTCAGCAATGCCTGTCTCTTCTACAC480.12932776505456015No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGGAC250.00331933421.00000211
GCCTTTT250.00331933421.00000218
CAATGCT8000.021.0000027
AGCTTTT250.00331933421.00000218
TGCGTGA250.00331933421.00000210
TGCGTCG250.00331933421.00000210
GTCGTTT250.00331933421.00000217
TGCCGGG558.29823E-921.00000210
TGCCGGA250.00331933421.00000210
TGCCGAG250.00331933421.00000210
CGCTTTT558.29823E-921.00000218
TGCGATG250.00331933421.00000210
TGCTGTG250.00331933421.00000210
TGCGAGG507.079143E-821.00000210
CCGTTTT558.29823E-921.00000218
CGGATTT250.00331933421.00000217
ATGCGGT1100.021.0000029
ATGCGAG950.021.0000029
AACTTTT250.00331933421.00000218
GCTGTTT250.00331933421.00000217
Produced by FastQC (version 0.11.2)