FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate8_6.35100000060739.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate8_6.35100000060739.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19875
Sequences flagged as poor quality0
Sequence length27
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGATCGTAGCCTGTCTCTTATACACA2221.1169811320754717No Hit
CTGATCGTAGCACCTGTCTCTTATACA710.35723270440251575No Hit
CTGATCGTAGCAGCTGTCTCTTATACA400.2012578616352201No Hit
CTGATCGTAGCATCTGTCTCTTATACA310.1559748427672956No Hit
CTGATCGTAGCAGGGGGGGGTTTTTTT250.12578616352201258No Hit
CTGATCGTAGCCTGTCTCTTATACCCA230.11572327044025157No Hit
CTGATCGTAGCCTGTCTCTTCTACACA210.10566037735849057No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCTCG651.0004442E-1021.0505071
CTGATCG16750.021.0505051
CGGATCG950.021.0505051
CTGAACG303.688334E-421.0505051
CTTATCG354.2372732E-521.0505051
CCGATCG506.4987034E-821.0505051
TTTCTGT850.020.99748221
TGATCGT16800.020.9974822
TTCTGTC557.734343E-920.99748221
GGATTTT404.98816E-620.99748218
TAGCATT850.020.9974828
TAGCATG850.020.9974828
TAGCATC1150.020.9974828
GAGTTTT651.03682396E-1020.99748218
ACTTTTT1100.020.99748219
TAGCAGG4600.020.9974828
TAGCAGA1050.020.9974828
TAGCACG1500.020.9974828
TAGCACC751.8189894E-1220.9974828
TAGCACA751.8189894E-1220.9974828
Produced by FastQC (version 0.11.2)