FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate8_51.35100000060cc7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate8_51.35100000060cc7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1250
Sequences flagged as poor quality0
Sequence length27
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGGAACAGAGTCTGTCTCTTATACAC141.1199999999999999No Hit
GAGGAACAGAGTCTGTCTCTTCTACAC50.4No Hit
GAGGAACAGAGTTGTCACAGTTTTTTT30.24No Hit
GAGGAACAGAGTCGGGGAGGTTTTTTT30.24No Hit
GAGGAACAGAGCTGTCTCTTATACACA30.24No Hit
GAGGAACAGAGTCGTGAGCATTTTTTT20.16No Hit
GAGGAACAGAGTCGGCCGCGTTTTTTT20.16No Hit
GAGGAACAGAGTGAGTCCCGTTTTTTT20.16No Hit
GAGGAACAGAGTATAATGGGTTTTTTT20.16No Hit
GAGGAACAGAGTTGTGGGGGTTTTTTT20.16No Hit
GAGGAACAGAGTTGGGAACGTTTTTTT20.16No Hit
GAGGAACAGAGTTTATCGAGTTTTTTT20.16No Hit
GAGGAACAGAGTAGCTGTCTCTTATAC20.16No Hit
GAGGAACAGAGTTGGGGTAATTTTTTT20.16No Hit
GAGGCACAGAGTCTGTGTGCTTTTTTT20.16No Hit
GAGGAACAGAGTGCCCCATGTTTTTTT20.16No Hit
GAGGAACAGAGTAGGTGCTGTCTCTTA20.16No Hit
GAGGAACAGAGTGGGGAGGGTTTTTTT20.16No Hit
GAGGAACAGAGTGGCTGTCTCTTATAC20.16No Hit
GAGGAACAGAGTAGAGGCTGTCTCTTA20.16No Hit
GAGGAACAGAGTGTCGGTACTTTTTTT20.16No Hit
GAGGAACAGAGTCGGAACGGTTTTTTT20.16No Hit
GAGGAACAGAGTCTCAGACCTTTTTTT20.16No Hit
GAGGAACAGAGTCGGACAACTTTTTTT20.16No Hit
GAGGAACAGAGTGACGCCCTGTCTCTT20.16No Hit
GAGGAACAGAGTGGGGCAAGTTTTTTT20.16No Hit
GAGGAACAGAGTGGGCGCTGTCTCTTC20.16No Hit
GAGGAACAGAGTTGCCTGGCTTTTTTT20.16No Hit
GAGGAACAGAGTGGTGGTGATTTTTTT20.16No Hit
GAGGAACAGAGTGCCCGACCTTTTTTT20.16No Hit
GAGGAACAGAGTCCGCCAGCTTTTTTT20.16No Hit
GAGGAACAGAGTGGAAGATCTTTTTTT20.16No Hit
GAGGAACAGAGTGCTGTCTCTTATACA20.16No Hit
GAGGAACAGAGTTGGCCCCATTTTTTT20.16No Hit
GAGGAACAGAGTTTTACAAATTTTTTT20.16No Hit
GAGGAACAGAGTGTTGGATCTTTTTTT20.16No Hit
GAGGAACAGAGTATCGACCGTTTTTTT20.16No Hit
GAGGAACAGAGTATATTCTGTCTCTTA20.16No Hit
GAGGAACAGAGTGTACGAAGTTTTTTT20.16No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAGAGT1200.021.0000026
GAACAGA1200.021.0000024
AACAGAG1200.021.0000025
ATTTTTT301.6740938E-421.00000220
GGAACAG1200.021.0000023
GAGGAAC1150.021.01
AGGAACA1150.021.02
CAGAGTA351.44566875E-521.07
GTTTTTT250.001853412620.99999820
CAGAGTG554.7293724E-1020.9999987
CAGAGTC250.001853412620.9999987
CTTTTTT300.005364295617.50000220
TTTTTTT1150.015.52173921
Produced by FastQC (version 0.11.2)