FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate8_47.35100000060c41.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate8_47.35100000060c41.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47118
Sequences flagged as poor quality0
Sequence length27
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGCAGACTGGAGGCTGTCTCTTATAC870.1846428116643321No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATGTG354.4834946E-521.00000210
GGATGTA354.4834946E-521.00000210
GGATGAA354.4834946E-521.00000210
GAGCGGG354.4834946E-521.00000211
GCCTTTT354.4834946E-521.00000218
TTGCAGA354.4834946E-521.0000021
ACGTTTT701.4551915E-1121.00000218
GGATTGG354.4834946E-521.00000210
ACATTTT558.443749E-921.00000218
TGGAGCC558.443749E-921.0000029
AGTGTTT354.4834946E-521.00000217
CTGGAAT2150.021.0000028
CTGGAAC1700.021.0000028
CTGGAAA2800.021.0000028
TGGATTC558.443749E-921.0000029
GCAGACT42600.021.0000023
TGGATGA1100.021.0000029
TGGACCT558.443749E-921.0000029
TGGAATC354.4834946E-521.0000029
GGAGGCA354.4834946E-521.00000210
Produced by FastQC (version 0.11.2)