FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate8_44.35100000060be2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate8_44.35100000060be2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44748
Sequences flagged as poor quality0
Sequence length27
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTCTGT354.4419477E-521.02127621
TTTTCTG250.00331034921.02127621
GTTTCTG250.00331034921.02127620
TCTTATA250.00331034921.02127621
CTCTGAG3250.020.9977658
AATTTTT1500.020.99776519
TCTGGCC751.8189894E-1220.9977659
ACTCTGG17850.020.9977657
TCTGAGC751.8189894E-1220.9977659
TCTGAAG751.8189894E-1220.9977659
TTAGACA1350.020.9977631
AGGGGTT405.2260857E-620.99776316
CAGCCAC701.4551915E-1120.9977632
CTCTGGT4000.020.9977638
CTCTGGG7000.020.9977638
CTCTGGC3450.020.9977638
CTCTGGA3400.020.9977638
CTCTGCT2050.020.9977638
CTCTGCG3050.020.9977638
CTCTGCC1600.020.9977638
Produced by FastQC (version 0.11.2)