FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate8_42.35100000060baf.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate8_42.35100000060baf.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29287
Sequences flagged as poor quality0
Sequence length27
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAAGAGA250.003274601221.0308233
TCCCAGA303.7709778E-421.0308231
TCAAGAG250.003274601221.0308232
CCCAGAG303.7709778E-421.0308232
ACAGAGA303.7709778E-421.0308233
TACAGAG303.7709778E-421.0308232
TTCAAGA250.003274601221.0308231
TTACAGA303.7709778E-421.0308231
CCAGAGA26200.021.0308233
TTTCAGA651.09139364E-1021.0308231
TCCAGAG23750.021.0308232
GCCAGAG1800.021.0308232
TTCCAGA23750.021.0308231
TGCCAGA1800.021.0308231
TCAGAGA651.09139364E-1021.0308233
CCCGAGA1450.021.0308233
TTCAGAG651.09139364E-1021.0308232
TACCAGA354.359061E-521.0308231
ACCAGAG354.359061E-521.0308232
ACTGCTT354.417268E-520.9948739
Produced by FastQC (version 0.11.2)