FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate8_41.35100000060b92.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate8_41.35100000060b92.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41198
Sequences flagged as poor quality0
Sequence length27
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCAACCGATAGGCTGTCTCTTATACAC2200.5340065051701539No Hit
TCAACCGATAGGCTGTCTCTTCTACAC910.22088450895674547No Hit
TCAACCGATAGGGCTGTCTCTTATACA420.10194669644157484No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTACCG1350.021.0194661
TACCGAT1350.021.0194663
CTACCGA1350.021.0194662
AACCGAT37100.021.0194643
AACGATA609.713403E-1021.0194644
CAACCGA36500.021.0194642
CACCGAT900.021.0194643
ACCCGAT651.1459633E-1021.0194643
TCAACCG36650.021.0194641
ACCGATA39350.021.0194644
CCACCGA900.021.0194642
TCAAACG609.713403E-1021.0194641
TAACCGA651.1459633E-1021.0194642
CACCCGA651.1459633E-1021.0194642
CCCGATA651.1459633E-1021.0194644
TCCACCG900.021.0194641
TCACCCG651.1459633E-1021.0194641
ACGATAG609.713403E-1021.0194645
TTAACCG651.1459633E-1021.0194641
CAAACGA609.713403E-1021.0194642
Produced by FastQC (version 0.11.2)