FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate8_40.35100000060b78.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate8_40.35100000060b78.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37708
Sequences flagged as poor quality0
Sequence length27
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGAGCTTGACCTGTCTCTTATACAC1530.40574944308899963No Hit
GTTGAGCTTGACCTGTCTCTTCTACAC540.14320568579611753No Hit
GTTGAGCTTGACGCTGTCTCTTATACA460.12199002864113714No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCTTTT303.8371893E-421.00000218
TATATTT303.8371893E-421.00000217
TGACTTA303.8371893E-421.0000029
TATACAC303.8371893E-421.00000221
GACAGTG303.8371893E-421.00000210
TGACCGA405.1853476E-621.0000029
AGGATTT405.1853476E-621.00000217
GTCGTTT303.8371893E-421.00000217
GGCGTTT303.8371893E-421.00000217
TGACCCC405.1853476E-621.0000029
TGACCAC303.8371893E-421.0000029
GGTCTTT303.8371893E-421.00000217
GAAGTTT303.8371893E-421.00000217
GACGGTC303.8371893E-421.00000210
GACGGTA609.749783E-1021.00000210
AGGGCTG303.8371893E-421.00000217
ATGGTTT405.1853476E-621.00000217
AGGGTTT303.8371893E-421.00000217
GACGGCT405.1853476E-621.00000210
TGACAGC405.1853476E-621.0000029
Produced by FastQC (version 0.11.2)