FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate8_36.35100000060af0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate8_36.35100000060af0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences83703
Sequences flagged as poor quality0
Sequence length27
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGAACGACAGCTGTCTCTTATACACA3090.36916239561306047No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTCTGT456.1440915E-721.03411120
TAACGAC1750.020.9964183
ATAACGA1750.020.9964182
AGGGGTT250.003357344320.99641616
ACTGTTT354.5434845E-520.99641617
ACAGTGT7450.020.9964168
ACAGTGG15300.020.9964168
TTGTCTG303.8962552E-420.99641618
ACAGTGA5450.020.9964168
TGGTTTT1800.020.99641618
GTATGTG303.8962552E-420.99641611
ACAGTCC3400.020.9964168
ACAGTCA3250.020.9964168
TCCTTTT405.320144E-620.99641618
ACAGTAA2700.020.9964168
ACGCTTT303.8962552E-420.99641617
GCCTTTT800.020.99641618
CAGTCTA354.5434845E-520.9964169
CAGTTTG1400.020.9964169
GTCATTT250.003357344320.99641617
Produced by FastQC (version 0.11.2)