FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate8_35.35100000060ad6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate8_35.35100000060ad6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54684
Sequences flagged as poor quality0
Sequence length27
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACAGAAGGACCTGTCTCTTATACACA4600.8411966937312559No Hit
CACAGAAGGACTGGGGGCTGTCTCTTA750.13715163484748738No Hit
CACAGAAGGACTGGGGCTGTCTCTTAT690.1261795040596884No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGTTTT950.021.00000218
ACTGCGT405.2566775E-621.00000210
ACTGCGG800.021.00000210
ACTGCAG405.2566775E-621.00000210
ACTCGTG405.2566775E-621.00000210
ACTGTAG405.2566775E-621.00000210
CAGTTTT558.520146E-921.00000218
ACTCGGG800.021.00000210
AAATTTT405.2566775E-621.00000218
TATACAC405.2566775E-621.00000220
ACTTGGG800.021.00000210
ACTTGCG405.2566775E-621.00000210
GGACTCC1600.021.0000028
CACCGAA1600.021.0000021
GGATTTT800.021.00000218
CGAAGGA1600.021.0000024
TAGTTTT405.2566775E-621.00000218
CTAGGGG405.2566775E-621.00000211
ACCGAAG1600.021.0000022
CTGGAGG405.2566775E-621.00000211
Produced by FastQC (version 0.11.2)