FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate8_34.35100000060abc.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate8_34.35100000060abc.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27245
Sequences flagged as poor quality0
Sequence length27
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTCGTCTCGATCTGTCTCTTATACAC1730.6349788952101303No Hit
ACTCGTCTCGATCTGTCTCTTCTACAC460.1688383189576069No Hit
ACTCGTCTCGATGCTGTCTCTTATACA310.11378234538447422No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTTTT651.05501385E-1021.05535118
GGGTTTT1300.021.05535118
GCCTTTT303.7317575E-421.05535118
CAGTTTT250.003246545821.05535118
AAATTTT404.981599E-621.05535118
ACGTTTT354.305223E-521.05535118
GGCGTTT250.003246545821.05535117
TTTCTGT701.2732926E-1121.05535121
GTTCTGT354.305223E-521.05535120
TTATTTT506.7126166E-821.05535118
CGCTTTT303.7317575E-421.05535118
GCGGTTT250.003246545821.05535117
AGGGTTT354.305223E-521.05535117
TTCTGTC609.094947E-1021.05535121
GGATTTT850.021.05535118
CAATTTT354.305223E-521.05535118
GTTTCTG354.305223E-521.05535120
GGTTCTG250.003246545821.05535119
GGTTTTT2900.021.05535119
CATTTTT1750.021.05535119
Produced by FastQC (version 0.11.2)