FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate8_33.35100000060a93.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate8_33.35100000060a93.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19546
Sequences flagged as poor quality0
Sequence length27
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AACGGAATGGCCTGTCTCTTATACACA800.4092909035096695No Hit
AACGGAATGGCTCCTGTCTCTTATACA440.22510999693031825No Hit
AACGGAATGGCTGCTGTCTCTTATACA430.21999386063644735No Hit
AACGGAATGGCTGTCTCTTATACACAT380.19441317916709303No Hit
AACGGAATGGCTCTGTCTCTTATACAC340.17394863399160954No Hit
AACGGAATGGCTACTGTCTCTTATACA230.11767113475902999No Hit
AACGGAATGGCTGGGGGCTGTCTCTTA220.11255499846515912No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGTTTT354.312124E-521.018
AAATTTT303.745833E-421.018
ATGGCTT3650.021.07
TGTTTTT1050.021.019
GCTGGGC354.312124E-521.010
GCTGGCG354.312124E-521.010
GGCTGGG1950.021.09
GGCTGGC608.913048E-1021.09
GGCTGCG608.913048E-1021.09
GGCTGAT303.745833E-421.09
GGCTGAG751.8189894E-1221.09
GGCTGAA354.312124E-521.09
TCGGAAT651.03682396E-1021.02
GAATGGC19000.021.05
ATGGAAT354.312124E-521.02
CGGAATG18350.021.03
GCTGCGA303.745833E-421.010
ATCGGAA651.03682396E-1021.01
CCTTTTT303.745833E-421.019
GGCTCGT303.745833E-421.09
Produced by FastQC (version 0.11.2)