FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate8_31.35100000060a50.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate8_31.35100000060a50.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39219
Sequences flagged as poor quality0
Sequence length27
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGACATCGCAAAAAAAAAAAAAAAAA420.10709095081465617No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTTTT850.021.00000218
TATATTT354.4587716E-521.00000217
GCAAGGA701.2732926E-1121.0000029
GCAAGCG950.021.0000029
GCAAGAA701.2732926E-1121.0000029
TATGTTT354.4587716E-521.00000217
GTTTCTG354.4587716E-521.00000220
CGGATTT354.4587716E-521.00000217
GCAACTC354.4587716E-521.0000029
GCAACTA354.4587716E-521.0000029
GCAATTG850.021.0000029
GAGTTTT850.021.00000218
GCAACGT850.021.0000029
GCAACAG701.2732926E-1121.0000029
GCAATAA701.2732926E-1121.0000029
CGGGTTT354.4587716E-521.00000217
CAATAGA354.4587716E-521.00000210
ATTGTTT354.4587716E-521.00000217
GCAAAGG1300.021.0000029
GCAAACA354.4587716E-521.0000029
Produced by FastQC (version 0.11.2)