FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate8_28.351000000609f1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate8_28.351000000609f1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25532
Sequences flagged as poor quality0
Sequence length27
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAGGACGAGATCTGTCTCTTATACAC1610.6305812313958954No Hit
AGAGGACGAGCTGTCTCTTATACACAT1040.40733197556008144No Hit
AGAGGACGAGATCTGTCTCTTCTACAC700.27416575278082406No Hit
AGAGGACGAGCTGTCTCTTATCCACAT500.19583268055773148No Hit
AGAGGACGAGATGCTGTCTCTTATACA360.14099953000156668No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTTTT850.021.03143518
TGCATTT250.003262582221.03143517
GGGTTTT1000.021.03143518
CGCCTTT250.003262582221.03143517
GTCGTTT250.003262582221.03143517
CTCTTTT250.003262582221.03143518
GCGGTTT354.329648E-521.03143517
CATTTTT1050.021.03143519
GAGTTTT250.003262582221.03143518
ACTTTTT557.854396E-921.03143519
CTGGTTT354.329648E-521.03143517
GTCTTTT354.329648E-521.03143518
CCTTTTT1350.021.03143519
GAATTTT354.329648E-521.03143518
GCGTTTT850.021.03143518
ATGTTTT250.003262582221.03143518
AGGTTTT250.003262582221.03143518
ACCTTTT250.003262582221.03143518
GGGGTTT354.329648E-521.03143517
CGGTTTT557.854396E-921.03143518
Produced by FastQC (version 0.11.2)