FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate8_27.351000000609ca.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate8_27.351000000609ca.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16649
Sequences flagged as poor quality0
Sequence length27
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCTGTC354.1722695E-521.05740221
ATTTCTG250.003191949621.05740220
ATTTTTT2300.021.05740220
CTTTTTT2850.021.05740220
TTTCTGT455.494603E-721.057421
TTGTTTT404.9134014E-620.99397718
TTTGACC250.003248706720.9939771
GTGTTTT701.0913936E-1120.99397718
CCAACGA16500.020.9939776
AACGATT800.020.9939778
AACGATG1050.020.9939778
AACGATA800.020.9939778
AACGAGT800.020.9939778
AACGAGA1600.020.9939778
AACGACT800.020.9939778
AACGACC557.516064E-920.9939778
ACCAACG15450.020.9939775
CGAGGGG701.0913936E-1120.99397710
CGAGGAG354.2714863E-520.99397710
ACGAGTG250.003248706720.9939779
Produced by FastQC (version 0.11.2)