FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate8_26.351000000609a1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate8_26.351000000609a1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28696
Sequences flagged as poor quality0
Sequence length27
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTGTCTGTGTCCTGTCTCTTATACAC3831.3346807917479788No Hit
ACTGTCTCTGTCTCTTATACACATCTC2730.9513521048229717No Hit
ACTGTCTGTGTCCTGTCTCTTCTACAC1230.42863116810705326No Hit
ACTGTCTCTGTCTCTTATACCCATCTC400.1393922497909116No Hit
ACTGTCTGTGTCCCTGTCTCTTATACA340.11848341232227488No Hit
ACTGTCTGTCTCTTATACACATCTCCG310.10802899358795652No Hit
ACTGTCTGTGTCTGTCTCTTATACACA310.10802899358795652No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTCTT1500.021.0000028
GTGTCCA1500.021.0000028
GTGTCAG2750.021.0000028
ACCGTCT751.8189894E-1221.0000021
CCGTCTG751.8189894E-1221.0000022
ACTTTTT1500.021.00000219
TTGTCTG354.404872E-521.02
ACTGCCT303.8060837E-421.01
GTGTCTC800.021.08
GTGTCTA1450.021.08
GTGTTTT900.021.018
GGGTTTT1200.021.018
GCCTTTT303.8060837E-421.018
CAGTTTT354.404872E-521.018
GTCACTC250.003301062221.010
GTCATTG303.8060837E-421.010
GTGTCGT2450.021.08
GTGTCGG3050.021.08
GTGTCGC1950.021.08
ATCTTTT303.8060837E-421.018
Produced by FastQC (version 0.11.2)