FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate8_24.3510000006096d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate8_24.3510000006096d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences92194
Sequences flagged as poor quality0
Sequence length27
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTAGTGTGGAGGCTGTCTCTTATAC5310.5759593899819945No Hit
GTGTAGTGTGGCTGTCTCTTATACACA4240.4598997765581274No Hit
GTGTAGTGTGGAGTCTGTCTCTTATAC2160.23428856541640453No Hit
GTGTAGTGTGGAGCCTGTCTCTTATAC2060.22344187257305248No Hit
GTGTAGTGTGGAAGCTGTCTCTTATAC2000.21693385686704122No Hit
GTGTAGTGTGGATGCTGTCTCTTATAC1610.1746317547779682No Hit
GTGTAGTGTGGAGACTGTCTCTTATAC1360.14751502266958802No Hit
GTGTAGTGTGGAGCTGTCTCTTATACA1260.13666832982623597No Hit
GTGTAGTGTGGACGCTGTCTCTTATAC1210.13124498340455995No Hit
GTGTAGTGTGGACCTGTCTCTTATACA1100.11931362127687269No Hit
GTGTAGTGTGGAACCTGTCTCTTATAC980.1062975898648502No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACACA354.526722E-521.01031721
CTTTTTT11750.021.01031520
GATGGGC354.5457287E-520.99891711
GATGGGA354.5457287E-520.99891711
TGCATTT354.5457287E-520.99891717
GGATGGC701.4551915E-1120.99891710
GGATGGA701.4551915E-1120.99891710
GGATGAC354.5457287E-520.99891710
GATATTT354.5457287E-520.99891717
GTACTTT354.5457287E-520.99891717
GGATCGG354.5457287E-520.99891710
GGATTAT354.5457287E-520.99891710
CGTAGTG1400.020.9989172
TACATTT354.5457287E-520.99891717
ATAATTT354.5457287E-520.99891717
GGATATA354.5457287E-520.99891710
CGGGTTT701.4551915E-1120.99891717
TCAGTTT354.5457287E-520.99891717
GGCCTTT354.5457287E-520.99891717
GACGTTG354.5457287E-520.99891711