FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate8_21.35100000060900.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate8_21.35100000060900.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48368
Sequences flagged as poor quality0
Sequence length27
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTTGAACTGGGGCTGTCTCTTATAC790.16333112801852465No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGAAC250.003333799321.0000023
TGGTTGG354.486674E-521.00000210
TGGCATG354.486674E-521.00000210
TGGCAGG354.486674E-521.00000210
GGCATTT250.003333799321.00000217
GGGTCGG250.003333799321.00000211
AGCTTTT250.003333799321.00000218
AATCTGT900.021.00000219
AATTTTT1000.021.00000219
AACTGTC900.021.00000219
AACTGGA9100.021.0000027
CTGGGGG2600.021.0000029
CTGGGGA1300.021.0000029
CTGGGCC1000.021.0000029
GGACTGT507.192648E-821.00000218
TGATCTG354.486674E-521.00000218
TTATTTT250.003333799321.00000218
TGATTTT456.129103E-721.00000218
CCTCGAA250.003333799321.0000022
TCGAACT250.003333799321.0000024
Produced by FastQC (version 0.11.2)