FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate8_20.351000000608e5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate8_20.351000000608e5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31523
Sequences flagged as poor quality0
Sequence length27
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTCTCCTACCTGTCTCTTATACACAT2990.9485137835865876No Hit
AGTCTCCTACGTCTGTCTCTTATACAC2690.8533451765377662No Hit
AGTCTCCTACCTGTCTCTTATCCACAT1100.34895155917901216No Hit
AGTCTCCTACGTCTGTCTCTTCTACAC960.3045395425562288No Hit
AGTCTCCTACGCTGTCTCTTATACACA800.2537829521301907No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTTTT800.021.02543818
GGGTTTT1350.021.02543818
AATTTTT651.10958354E-1021.02543819
TTATTTT651.10958354E-1021.02543818
CGCTTTT405.0864346E-621.02543818
GAGTTTT651.10958354E-1021.02543818
ACTTTTT800.021.02543819
TACACAT405.0864346E-621.02543821
CCATTTT455.91981E-721.02543818
CCTTTTT558.06358E-921.02543819
TCGTTTT455.91981E-721.02543818
AGATTTT455.91981E-721.02543818
TTGTTTT609.422365E-1021.02543618
GCCTTTT250.003285054421.02543618
GGCATTT250.003285054421.02543617
CCTGTTT303.7865128E-421.02543617
GGCGTTT303.7865128E-421.02543617
TGATTTT303.7865128E-421.02543618
AGGGTTT250.003285054421.02543617
GTATTTT506.9034286E-821.02543618
Produced by FastQC (version 0.11.2)