FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate8_2.351000000606a4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate8_2.351000000606a4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43169
Sequences flagged as poor quality0
Sequence length27
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTAGTGAGCTAGGGCTGTCTCTTATA630.1459380574023026No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTCTGT250.003307802821.02204120
AGCTACG3400.020.9976848
CTAGGTG850.020.99768410
TCCTTTT303.8525515E-420.99768318
GGGTTTT1500.020.99768318
GCCTTTT651.1641532E-1020.99768318
CAGTTTT405.2192863E-620.99768318
CTATAGA303.8525515E-420.99768310
TAGGAGT250.00333017720.99768311
TAGGAGG303.8525515E-420.99768311
TAGTGGG250.00333017720.99768311
GCTAGTG1500.020.9976839
GCTAGTA701.4551915E-1120.9976839
GATATTT250.00333017720.99768317
GCGATTT303.8525515E-420.99768317
GCTAGGG2650.020.9976839
GCTAGGC1350.020.9976839
GCTAGGA1650.020.9976839
AGGATTT405.2192863E-620.99768317
GGCGCTT250.00333017720.99768316
Produced by FastQC (version 0.11.2)