FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate8_17.35100000060888.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate8_17.35100000060888.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8161
Sequences flagged as poor quality0
Sequence length27
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AACGTGCGTATGCTGTCTCTTATACAC570.6984438181595393No Hit
AACGTGCGTATGGGCTGTCTCTTATAC300.3676020095576522No Hit
AACGTGCGTATGCTGTCTCTTCTACAC270.33084180860188706No Hit
AACGTGCGTATGGCTGTCTCTTATACA260.3185884082832986No Hit
AACGTGCGTATCTGTCTCTTATACACA210.2573214066903566No Hit
AACGTGCGTATGTCTGTCTCTTATACA170.2083078054160029No Hit
AACGTGCGTATGACCTGTCTCTTATAC160.19605440509741454No Hit
AACGTGCGTATGCCTGTCTCTTATACA150.1838010047788261No Hit
AACGTGCGTATGGCCTGTCTCTTATAC130.1592942041416493No Hit
AACGTGCGTATGAGCTGTCTCTTATAC100.12253400318588407No Hit
AACGTGCGTATGCCCTGTCTCTTATAC90.11028060286729569No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGTTTT353.925526E-521.00000218
TGGTTTT250.00310400521.00000218
GTATGTG556.159098E-921.0000028
GTATGGC556.159098E-921.0000028
GTATGCT353.925526E-521.0000028
GTATGCC353.925526E-521.0000028
GGGTTTT250.00310400521.00000218
GGCGTTT250.00310400521.00000217
TGCGTAT8000.021.0000025
TATGCTG250.00310400521.0000029
TGTTTTT556.159098E-921.00000219
GTCTGTC250.00310400521.00000220
ACTTTTT505.5246346E-821.00000219
AACTTTT353.925526E-521.00000218
AGTGCGT250.00310400521.0000023
TATTTTT505.5246346E-821.00000219
GATTTTT556.159098E-921.00000219
GCGTATG8000.021.0000026
AAGTGCG250.00310400521.0000022
AAAGTGC250.00310400521.0000021
Produced by FastQC (version 0.11.2)