FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate8_14.35100000060838.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate8_14.35100000060838.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22638
Sequences flagged as poor quality0
Sequence length27
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGCTCTGTTGCTGTCTCTTATACACA1940.8569661630886121No Hit
ATGCTCTGTTGCGGCTGTCTCTTATAC550.24295432458697766No Hit
ATGCTCTGTTGCGGGGCTGTCTCTTAT490.21645021645021645No Hit
ATGCTCTGTTGCGCCTGTCTCTTATAC300.13252054068380598No Hit
ATGCTCTGTTGCAGCTGTCTCTTATAC300.13252054068380598No Hit
ATGCTCTGTTGCCTGTCTCTTATACAC280.12368583797155226No Hit
ATGCTCTGTTGCACCTGTCTCTTATAC240.10601643254704478No Hit
ATGCTCTGTTGCTGTCTCTTCTACACA230.10159908119091793No Hit
ATGCTCTGTTGCGAGGCTGTCTCTTAT230.10159908119091793No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTGTTG405.0343624E-621.0000025
GGCGTTT405.0343624E-621.00000217
GTATTTT405.0343624E-621.00000218
CCGTTTT405.0343624E-621.00000218
ATGCCCT405.0343624E-621.0000021
ATGTTTT405.0343624E-621.00000218
AGGTTTT405.0343624E-621.00000218
TTGCGTG950.021.0000029
TTGCGGT651.05501385E-1021.0000029
TTGCGCT405.0343624E-621.0000029
TTGCGCG651.05501385E-1021.0000029
TTGCGCA405.0343624E-621.0000029
GTTGCTC800.021.0000028
GGTGTTT405.0343624E-621.00000217
TGCGGAG405.0343624E-621.00000210
GTTGCCG1900.021.0000028
GTTGCCA651.05501385E-1021.0000028
GTTGCAT651.05501385E-1021.0000028
GTTGCAC800.021.0000028
GTTGCAA950.021.0000028
Produced by FastQC (version 0.11.2)