FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate7_96.35100000060660.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate7_96.35100000060660.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16978
Sequences flagged as poor quality0
Sequence length27
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAAGCGTGGAACCTGTCTCTTATACAC2421.4253740134291437No Hit
TAAGCGTGGAACCTGTCTCTTCTACAC740.43585816939568855No Hit
TAAGCGTGGAACTGTCTCTTATACACA220.12957945576628577No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGTTTT303.7177012E-421.00000218
AACGTAG303.7177012E-421.00000210
AGCTTTT303.7177012E-421.00000218
AACTGAG303.7177012E-421.00000210
GAACGGG1200.021.0000029
GAACGGC303.7177012E-421.0000029
ACTTTTT751.8189894E-1221.00000219
GGGGGTT303.7177012E-421.00000216
GAACCTT303.7177012E-421.0000029
GAACCTG751.8189894E-1221.0000029
GAACTTG303.7177012E-421.0000029
GAACTGA608.6765795E-1021.0000029
GAACTAG303.7177012E-421.0000029
TCGTTTT303.7177012E-421.00000218
ACGGGAG303.7177012E-421.00000211
GAACATT303.7177012E-421.0000029
GAACACG303.7177012E-421.0000029
TGGGTTT303.7177012E-421.00000217
TGGAACA3000.021.0000027
GGAACGT1500.021.0000028
Produced by FastQC (version 0.11.2)