FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate7_94.3510000006063a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate7_94.3510000006063a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences95617
Sequences flagged as poor quality0
Sequence length27
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TACTGCAAGGACTGTCTCTTATACACA2960.30956838219145133No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTTTTC250.003339067621.01989620
AGGGGTG751.8189894E-1220.9979111
GGAGATA1050.020.997919
CTACAAG1050.020.997913
GGAGACG1050.020.997919
GGAGACC456.2662184E-720.997919
GGAGACA751.8189894E-1220.997919
GGGTTTC456.2662184E-720.9979118
CAGTTTT456.2662184E-720.9979118
CATTTTT2100.020.9979119
AGGGATG456.2662184E-720.9979111
TACAAGG1050.020.997914
TGCTTTT751.8189894E-1220.9979118
GTCTTTT456.2662184E-720.9979118
TACTGCA89100.020.997911
ATGTTTT1050.020.9979118
CGTGTTT456.2662184E-720.9979117
TGGGTTT751.8189894E-1220.9979117
ACAAGGA1050.020.997915
GAGAGAG751.8189894E-1220.9979110
Produced by FastQC (version 0.11.2)