FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate7_93.35100000060610.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate7_93.35100000060610.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31195
Sequences flagged as poor quality0
Sequence length27
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTTTT701.2732926E-1121.00000218
AGCTTTT558.169081E-921.00000218
ACTCGAC701.2732926E-1121.0000022
ACGCTAT6650.021.0000027
ATCTCGA26200.021.0000021
GCTAGTC701.2732926E-1121.0000029
GCTAGTA558.169081E-921.0000029
GCTAGAT354.4208893E-521.0000029
GCTAGAA354.4208893E-521.0000029
CTCTTTT558.169081E-921.00000218
TTCGACG558.169081E-921.0000023
CCGTTTT701.2732926E-1121.00000218
GAGTTTT558.169081E-921.00000218
GCTATTA354.4208893E-521.0000029
CTAGGGT354.4208893E-521.00000210
CTAGGGG558.169081E-921.00000210
GCTACGG558.169081E-921.0000029
CGGGTTT354.4208893E-521.00000217
CTAGGAT354.4208893E-521.00000210
GCTACCA354.4208893E-521.0000029
Produced by FastQC (version 0.11.2)