FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate7_92.351000000605f5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate7_92.351000000605f5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61991
Sequences flagged as poor quality0
Sequence length27
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCACTCT59850.021.0121175
TTAGACA1600.021.0121151
CAGCCAC900.021.0121152
AGCCACT56500.021.0121153
TTCGCCA2100.021.0121151
TAAGCCA558.518327E-921.0121151
TAGCCAC51600.021.0121152
GAGCCAC3550.021.0121152
GACACTC1600.021.0121154
TCAGCCA900.021.0121151
TCGCCAC2100.021.0121152
GCCACTC59800.021.0121154
AAGCCAC558.518327E-921.0121152
AGACACT1600.021.0121153
TAGACAC1600.021.0121152
TTTGCCA1200.021.0121151
TTGCCAC1200.021.0121152
AGTTTTT2750.020.9951619
CTCTGTA2750.020.995168
CTCTGGT4550.020.9951578
Produced by FastQC (version 0.11.2)