FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate7_91.351000000605db.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate7_91.351000000605db.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37115
Sequences flagged as poor quality0
Sequence length27
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAGCTGGATGCTGTCTCTTATACACA3430.9241546544523779No Hit
CTAGCTGGATGTCTGTCTCTTATACAC1190.3206250841977637No Hit
CTAGCTGGATCTGTCTCTTATACACAT600.1616597063182002No Hit
CTAGCTGGATGTGGGCTGTCTCTTATA440.11855045130001347No Hit
CTAGCTGGATGCTGTCTCTTCTACACA410.11046746598410347No Hit
CTAGCTGGATGTGGGGGGGGTTTTTTT380.10238448066819344No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTGGA1000.021.0000023
GTGTTTT1000.021.00000218
GCCTTTT250.00331933421.00000218
AGCTTTT558.29823E-921.00000218
TGCGTTT250.00331933421.00000217
CCAGTTT250.00331933421.00000217
AGGATTT250.00331933421.00000217
TGTCTGG250.00331933421.00000210
TTATTTT507.079143E-821.00000218
TTTTCTG250.00331933421.00000221
GATGTAC1000.021.0000028
TTTTTCT250.00331933421.00000221
ACATTTT250.00331933421.00000218
GGGAGTT250.00331933421.00000216
ATGTGTC250.00331933421.0000029
ATGTGTA1000.021.0000029
GGGAGGT250.00331933421.00000215
ATGTGGT1100.021.0000029
CGGGTTT558.29823E-921.00000217
ATGTGGA507.079143E-821.0000029
Produced by FastQC (version 0.11.2)