FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate7_88.35100000060561.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate7_88.35100000060561.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46528
Sequences flagged as poor quality0
Sequence length27
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGAGCTTGACCTGTCTCTTATACAC2730.5867434662998624No Hit
GTTGAGCTTGACCTGTCTCTTCTACAC830.17838720770288857No Hit
GTTGAGCTTGACGCTGTCTCTTATACA630.13540233837689133No Hit
GTTGAGCTTGACGGCTGTCTCTTATAC530.11390990371389272No Hit
GTTGAGCTTGACCCTGTCTCTTATACA510.10961141678129298No Hit
GTTGAGCTTGACGGGGGGGGTTTTTTT500.10746217331499312No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCTTTT609.822543E-1021.01722318
ATCTTTT405.1915813E-621.01722318
TGCGTTT354.4535467E-521.01722317
TATACAC303.8345432E-421.01722321
GATATTT354.4535467E-521.01722317
AATTTTT1700.021.01722319
GGCGTTT751.8189894E-1221.01722317
GGACTTT303.8345432E-421.01722317
TCGGTTT354.4535467E-521.01722317
TTATTTT751.8189894E-1221.01722318
TGATTTT800.021.01722318
GCGGTTT558.356437E-921.01722317
TGTTTTT4600.021.01722319
GTATTTT751.8189894E-1221.01722318
CGGATTT405.1915813E-621.01722317
TAGTTTT850.021.01722318
ACATTTT303.8345432E-421.01722318
GAGTTTT1150.021.01722318
ACTTTTT1950.021.01722319
AACTTTT405.1915813E-621.01722318
Produced by FastQC (version 0.11.2)