FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate7_85.35100000060511.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate7_85.35100000060511.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38829
Sequences flagged as poor quality0
Sequence length27
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAGTACCGCTGTCTCTTATACACA1600.4120631486775348No Hit
CTCTAGTACCGAGGCTGTCTCTTATAC500.12876973396172964No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAAGGC354.4276218E-521.01806610
ACCGAGA2200.021.0180668
ACCGACG3400.021.0180668
ACCGACA1700.021.0180668
CGAGCCG354.4276218E-521.01806610
CGACGTT354.4276218E-521.01806610
CCGAGAG701.2732926E-1121.0180669
CGAGAGG354.4276218E-521.01806610
CGATGCA354.4276218E-521.01806610
CGATGAG354.4276218E-521.01806610
CCGACTA354.4276218E-521.0180669
CCGATTC558.245479E-921.0180669
CCGATTA354.4276218E-521.0180669
CCGACGT701.2732926E-1121.0180669
CCGACGG1400.021.0180669
CCGACGC701.2732926E-1121.0180669
CCGATGA701.2732926E-1121.0180669
CCGACCG354.4276218E-521.0180669
CCGACAG701.2732926E-1121.0180669
CCGATAA354.4276218E-521.0180669
Produced by FastQC (version 0.11.2)