FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate7_84.351000000604e9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate7_84.351000000604e9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40144
Sequences flagged as poor quality0
Sequence length27
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGAACGACAGCTGTCTCTTATACACA2070.5156436827421284No Hit
CAGAACGACAGTGGCTGTCTCTTATAC540.13451574332403346No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAGTCA1100.021.0000028
ACAGTAT1100.021.0000028
ACAGTAG2600.021.0000028
ACAGTAC1700.021.0000028
CAGTCTT303.8431044E-421.0000029
CAGTCTA303.8431044E-421.0000029
CAGTTTG558.349161E-921.0000029
CAGTTTA303.8431044E-421.0000029
AGCTTTT303.8431044E-421.00000218
CAGTCGC609.804353E-1021.0000029
CAGTTGT651.1459633E-1021.0000029
CAGTCCC609.804353E-1021.0000029
CAGTCAT303.8431044E-421.0000029
TGCGTTT303.8431044E-421.00000217
GCGATTT303.8431044E-421.00000217
GGCGTTT609.804353E-1021.00000217
AGTAGGG651.1459633E-1021.00000210
CAGTAGT558.349161E-921.0000029
CAGTAGG1100.021.0000029
CAGTAGA609.804353E-1021.0000029
Produced by FastQC (version 0.11.2)