FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate7_83.351000000604cf.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate7_83.351000000604cf.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences70743
Sequences flagged as poor quality0
Sequence length27
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACAGAAGGACCTGTCTCTTATACACA5250.7421228955515033No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCAGAA2150.021.0233731
CACACAA250.003327355321.023371
CACAAAA354.485949E-521.023371
CACCGAA1950.021.023371
CATAGAA1350.021.023371
CACAGAA64300.021.023371
TAGAAGG1350.021.0084933
TTGTTTT800.021.00849318
TGCATTT303.8728805E-421.00849317
ACAAAAG354.5105495E-521.0084932
GGGTTTT3200.021.00849318
GCCTTTT507.2814146E-821.00849318
CAGTTTT651.200533E-1021.00849318
GGCATTT354.5105495E-521.00849317
ATCTTTT354.5105495E-521.00849318
TAAATTT250.00334106421.00849317
AGCTTTT651.200533E-1021.00849318
GGGTTCT250.00334106421.00849318
GAAATTT354.5105495E-521.00849317
AAATTTT751.8189894E-1221.00849318
Produced by FastQC (version 0.11.2)