FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate7_77.35100000060412.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate7_77.35100000060412.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences64160
Sequences flagged as poor quality0
Sequence length27
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGGTCCAAGCACTGTCTCTTATACAC5160.8042394014962593No Hit
AAGGTCCAAGCCTGTCTCTTATACACA4980.7761845386533666No Hit
AAGGTCCAAGCAGTGGTATCAACGCAG820.12780548628428925No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCTTTT405.2260457E-621.0249818
GTGTTTT1600.021.0249818
GCCTTTT1100.021.0249818
AATCTGT250.003322545421.0249819
AAATTTT250.003322545421.0249818
AATTTTT1900.021.0249819
ACGTTTT1200.021.0249818
TTTCTGT1250.021.0249821
TTATCTG250.003322545421.0249818
TTATTTT900.021.0249818
CTCTTTT405.2260457E-621.0249818
GTTTCTG558.472853E-921.0249820
TAGTTTT1050.021.0249818
TGCTTTT1100.021.0249818
CCATTTT651.1823431E-1021.0249818
GTCTTTT800.021.0249818
GGCTTTT1100.021.0249818
GATCTGT456.123446E-721.0249819
TAATTTT354.4749388E-521.0249818
CACTTTT507.194649E-821.0249818
Produced by FastQC (version 0.11.2)