FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate7_75.351000000603dd.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate7_75.351000000603dd.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58309
Sequences flagged as poor quality0
Sequence length27
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGACCA2950.021.0154512
TGCGACC2950.021.0154511
TCGACCA47300.021.015452
TTCGCCC3300.021.015451
TTCGTCC250.003327707521.015451
TGACCAA1400.021.015453
CGCCCAA3300.021.015453
TTCGACC47300.021.015451
TCGCCCA3300.021.015452
TACGACC1000.021.015451
TCGTCCA250.003327707521.015452
TTGACCA1400.021.015452
TCCGACC1000.021.015451
CCGACCA1000.021.015452
TAGACCA651.1823431E-1021.015452
CGTCCAA250.003327707521.015453
GGCATTT558.561983E-920.99742917
AACGACT2450.020.9974298
AACGAAG4400.020.9974298
TTTTCTG558.561983E-920.99742921
Produced by FastQC (version 0.11.2)