FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate7_74.351000000603b3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate7_74.351000000603b3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58831
Sequences flagged as poor quality0
Sequence length27
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTGTCTGTGTCCTGTCTCTTATACAC5580.9484795430980266No Hit
ACTGTCTCTGTCTCTTATACACATCTC5250.8923866668933045No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTTTT1450.021.01361818
ACGTTTT651.1823431E-1021.01361818
AGGATTT651.1823431E-1021.01361817
ATGTTTT1300.021.01361818
AGGTTTT2150.021.01361818
ACCTTTT651.1823431E-1021.01361818
AACGTTT250.003329728521.01361717
AAACTTT250.003329728521.01361717
TGCATTT250.003329728521.01361717
CCAATTT250.003329728521.01361717
GTGTTTT1800.021.01361718
ACGCTTT354.4854634E-521.01361717
CAGTTTT751.8189894E-1221.01361718
GGCATTT303.855791E-421.01361717
TAAATTT303.855791E-421.01361717
CACATTT250.003329728521.01361717
TATATTT354.4854634E-521.01361717
TGCGTTT354.4854634E-521.01361717
GCGATTT405.238331E-621.01361717
ATGATTT250.003329728521.01361717
Produced by FastQC (version 0.11.2)