FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate7_73.3510000006039a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate7_73.3510000006039a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46525
Sequences flagged as poor quality0
Sequence length27
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTGGTTCGTTGCTGTCTCTTATACAC880.1891456206340677No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTCTGT303.830567E-421.02045220
GTTTCTG354.448277E-521.0204520
GTTGATA609.949872E-1020.997859
TTGTTGG303.8585844E-420.9978510
GTTGAGT751.8189894E-1220.997859
GTTGAGC701.4551915E-1120.997859
GCCTTTT609.949872E-1020.9978518
GTTGACA456.1246647E-720.997859
TGGCAGG303.8585844E-420.9978511
ATCTTTT456.1246647E-720.9978518
GGGTTCG1900.020.997853
AAATTTT751.8189894E-1220.9978518
TTCGTTG46000.020.997856
TGCGTTT303.8585844E-420.9978517
GCGATTT456.1246647E-720.9978517
AATTTTT3250.020.9978519
ACGTTTT609.949872E-1020.9978518
TTGTAGG303.8585844E-420.9978510
GGCGTTT456.1246647E-720.9978517
GGACTTT303.8585844E-420.9978517
Produced by FastQC (version 0.11.2)