FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate7_71.35100000060357.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate7_71.35100000060357.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63041
Sequences flagged as poor quality0
Sequence length27
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGCTATCCCTGTCTCTTATACACA3120.49491600704303546No Hit
ATCTGCTATCCTGTCTCTTATACACAT1150.1824209641344522No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTCCT507.228482E-821.0142971
ATCTACT900.021.0142971
TTTTCTG250.00333172221.01429721
TTTTTCT405.2467312E-621.01429721
GTTTCTG250.00333172221.01429720
TACACAT303.85919E-421.01429721
ACCTGCT1000.021.0142971
ATATGCT850.021.0142971
ATTTGCT751.8189894E-1221.0142971
ATTCTGT558.514689E-921.01429720
ATTTCTG303.85919E-421.01429720
ATCTGTC850.021.01429720
ATCTGCT54650.021.0142971
AGCTGCT3150.021.0142971
TTTCTGT701.4551915E-1121.01429621
AACTGCT701.4551915E-1121.0142961
CTTTTTT10350.021.01429620
CTCTGTC354.490921E-521.01429620
CCGCGAG250.003347123320.9976210
AGGGGTT354.518558E-520.9976216
Produced by FastQC (version 0.11.2)