FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate7_70.3510000006033d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate7_70.3510000006033d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences75810
Sequences flagged as poor quality0
Sequence length27
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCAGAACTACCGCTGTCTCTTATACA2130.2809655718242976No Hit
GTCAGAACTACCCCTGTCTCTTATACA1810.23875478169106978No Hit
GTCAGAACTACCTCTGTCTCTTATACA790.10420788814140614No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTCTGT751.8189894E-1221.0125421
TTTTCTG354.5093926E-521.0125421
GTTTCTG507.284143E-821.0125420
GTTTTCT354.5093926E-521.0125420
TTATACA601.0149961E-921.0125421
TTAGAAC1600.020.9986822
ACCGGTG1250.020.99868210
ACCGGGT1150.020.99868210
ACCGGAG900.020.99868210
ACCGGAC456.228711E-720.99868210
GTCATTT405.3042295E-620.99868217
ATCTTTT405.3042295E-620.99868218
AGGATTT405.3042295E-620.99868217
GTCGTTT456.228711E-720.99868217
ACCACGG456.228711E-720.99868210
AGGGTTT456.228711E-720.99868217
ACCGTTG456.228711E-720.99868210
ACCGCGA456.228711E-720.99868210
ACCGTCG456.228711E-720.99868210
ACCAAGG456.228711E-720.99868210
Produced by FastQC (version 0.11.2)