FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate7_69.35100000060313.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate7_69.35100000060313.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences60015
Sequences flagged as poor quality0
Sequence length27
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTTGAACTGGGGCTGTCTCTTATAC1140.189952511872032No Hit
ACCTTGAACTGGGCCTGTCTCTTATAC610.10164125635257852No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC800.021.0300521
CTTTCTC507.156814E-821.0300521
CTTTTTT5050.021.0300520
TTGTTTT601.0040822E-921.0125118
TCCTTTT250.00333153121.0125118
GTGTTTT1450.021.0125118
GCCTTTT354.4892586E-521.0125118
AGCTTTT354.4892586E-521.0125118
AATCTGT601.0040822E-921.0125119
AAATTTT250.00333153121.0125118
AATTTTT2000.021.0125119
TGACTGT250.00333153121.0125118
CTCCTGT250.00333153121.0125118
TGATCTG250.00333153121.0125118
CGCTTTT701.4551915E-1121.0125118
CATCTGT354.4892586E-521.0125119
TGTTTTT3300.021.0125119
GTATTTT601.0040822E-921.0125118
CAATTTT507.2202965E-821.0125118
CCGTTTT303.8584354E-421.0125118
Produced by FastQC (version 0.11.2)